口腔鳞癌癌周淋巴管生成与颈淋巴结微转移

目的　探讨口腔鳞癌癌周淋巴管生成与颈淋巴结微转移的关系。方法　对 4 7例口腔鳞癌组织、10例正常口腔黏膜进行酶组织化学染色 ,检测癌周微淋巴管密度 ;对 10例正常淋巴结、4 7例口腔鳞癌患者的 35 5个淋巴结以高分子量细胞角蛋白AE3单抗为抗体的免疫组织化学方法测定。结果　口腔鳞癌癌周淋巴管密度为 (14 0 4± 6 92 )个 / 2 0 0倍视野下 ,显著高于健康对照组 (5 4 8±2 6 2 )个 / 2 0 0倍视野下 (P <0 0 0 1) ;4 7例患者中角蛋白阳性 2 3例 ,阳性率为 4 8 9% (2 3/ 4 7) ;以淋巴微转移阳性、阴性分组 ,阳性组淋巴管密度为 (16 94± 5 4 3)个 / 2 0 0倍视野下 ,阴性组淋巴管密度为 (11 2 6± 5 0 0 )个 / 2 0 0倍视野下 ,阳性组微淋巴管密度显著高于阴性组 (P <0 0 0 1)。结论　癌周淋巴管密度增高在颈淋巴结微转移中起着非常重要的作用。

The relationship between lymphangiogenesis and cervical lymph node micrometastasis in oral squamous cell carcinoma

OBJECTIVE: To probe the relationship between lymphangiogenesis and cervical lymph node micrometastasis in oral squamous cell carcinoma. METHODS: The microlymphatic vessel density was detected with enzyme histo-chemical method in 47 cases of oral squamous cell carcinoma and 10 cases of normal oral mucosa; 355 lymphnodes were detected with immunohistochemically using monoantibody AE3. RESULTS: The mean MLVD was 14.04 +/- 6.92 in tumor group, or 5.48 +/- 2.62 in normal group. The difference was (P < 0.001). The percentage of tumor with expression CK was 48.9%. The mean MLVD was 16.94 +/- 5.43 in CK positive group, or 11.26 +/- 5.00 in CK negative group, There was difference significant (P < 0.001). CONCLUSIONS: Lymphangiogenesis plays a key role in cervical lymph node micrometastasis of oral squamous cell carcinoma.