| 苯丁酸钠联合奥沙利铂对结肠癌细胞HT29的凋亡作用 |
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| 引用本文： | 莫正英,梁清乐,戴冀斌. 苯丁酸钠联合奥沙利铂对结肠癌细胞HT29的凋亡作用[J]. 郧阳医学院学报, 2009, 28(5) |
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| 作者姓名： | 莫正英 梁清乐 戴冀斌 |
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| 作者单位： | 莫正英(武汉大学医学部基础医学院,湖北,武汉430071;郧阳医学院附属太和医院肿瘤科,湖北,十堰442000);梁清乐(郧阳医学院附属太和医院肿瘤科,湖北,十堰442000);戴冀斌(武汉大学医学部基础医学院,湖北,武汉430071) |
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| 摘 要： | 目的:探讨苯丁酸钠(SPB)联合奥沙利铂(L-OHP)诱导HT29细胞的凋亡作用及其机制。方法:结肠癌HT29细胞分为空白对照组、奥沙利铂组、苯丁酸钠(1 mmoL/L)+奥沙利铂组、苯丁酸钠(2 mmoL/L)+奥沙利铂组,MTT法测定各组细胞存活率,流式细胞仪检测凋亡率;Western blot检测细胞中凋亡相关蛋白Caspase-3和核转录因子NK-κB的表达。结果:苯丁酸钠能降低奥沙利铂诱导的HT29细胞存活率,随着其浓度增高,细胞凋亡率逐渐升高(P<0.01),细胞中Caspase-3蛋白和NK-κB蛋白表达则逐渐降低(P<0.05),均呈浓度依赖性。结论:苯丁酸钠(SPB)联合奥沙利铂(L-OHP)可诱导HT29细胞凋亡,其机制可能与抑制Caspase-3和NK-κB信号传导通路有关。
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| 关 键 词： | HT29细胞 苯丁酸钠 奥沙利铂 Caspase-3 NK-κB |
The Mechanism of Sodium Phenylbutyrate Enhances Apoptosis of HT29 Cells Induced by Oxaliplatin |
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| Affiliation: | Zheng-Ying[1] HANG Qing-le[2] DAI Ji-bin[3] |
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| Abstract: | objective To investigate the effects of sodium phenylbutyrate on oxaliplatin induced apoptosis in HT29 cells and its mechanism.Methods The HT29 cells were divided into four groups,Cell viability was tested by MTT assay and apoptotic ratio were measured by flow cytometry.The expressions of Caspase-3 and NK-κB proteins were tested by Western Blotting.Results The different concentrations of SPB inhibited the cell viability and enhanced the apoptosis based on L-OHP induced HT29 cells.The level of Caspase-3 and NK-κB were decreased with the SPB concentration rising.There was concentration dependent of the SPB.Conclusion SPB can enhance L-OHP induced apoptosis in HT29 cells and inhibit the expression of Caspase-3 and NK-κB which might be the possible signaling pathway of inducing apoptosis. |
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| Keywords: | Caspase-3 |
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