| 小鼠生精干细胞的初步分离与纯化 |
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| 引用本文： | 贾全安,张茨,王玲珑. 小鼠生精干细胞的初步分离与纯化[J]. 中国医师杂志, 2003, 0(Z1): 13-14 |
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| 作者姓名： | 贾全安 张茨 王玲珑 |
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| 作者单位： | 武汉大学人民医院泌尿外科,湖北,武汉,430060 |
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| 基金项目： | 湖北省教育厅资助项目(2000B03008) |
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| 摘 要： | 目的 寻求一种获取小鼠的生精干细胞的简单方法。方法 将新生小鼠睾丸采用改良的两步消化法消化 ,并通过贴壁分离方法纯化。结果 通过该方法能够获取较高纯度 ( 95 % )的A型精原细胞 ,并通过生精干细胞移植实验证实其有效性。结论 采用该方法对生精干细胞进行初步的分离和纯化是简单有效的
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| 关 键 词： | 生精干细胞 分离 纯化 |
| 修稿时间： | 2003-04-29 |
Preliminary Isolation and Purification of Spermatogonial Stem Cells |
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| JIA Quan-an,ZHANG Ci,WANG Ling-long. Preliminary Isolation and Purification of Spermatogonial Stem Cells[J]. Journal of Chinese Physician, 2003, 0(Z1): 13-14 |
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| Authors: | JIA Quan-an ZHANG Ci WANG Ling-long |
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| Affiliation: | JIA Quan-an,ZHANG Ci,WANG Ling-long.Department of Urology,Renmin Hospital of Wuhan University,Wuhan,430060,China |
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| Abstract: | Objective To obtain a simple method to isolate and purify spermatogonia stem cells. Methods The testis of newborn mouse were digested by modified two step digest method, and the type A spermatogonia cells were separated by different adhesive time with Sertoli cells. Results High concentration (95%) purified type A spermatogonia cells were acquired, and the spermatogonia transplanting experiment demonstrated the efficiency of the method.Conclusions This method is easy and efficient to isolation and purification of spermatogonial stem cells. |
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| Keywords: | Spermatogonial stem cells Isolation Purificaiton |
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