间歇低氧诱发兔动脉粥样硬化形成中差异基因分析

目的研究间歇低氧诱发动脉粥样硬化形成的重要作用基因。方法构建间歇低氧动脉粥样硬化兔模型,提取兔腹主动脉总RNA,逆转录制备杂交探针,与人的心血管疾病基因列阵Ⅱ(HS-038)芯片杂交后分析差异表达基因,采用逆转录聚合酶链反应验证差异基因。结果共有187条基因表达水平显著改变;涉及凋亡、蛋白质合成、癌基因、抑癌基因、信号传导基因、发育相关基因、炎症等诸多方面,其中环氧合酶2mRNA的差异表达与腹主动脉内膜病理学变化具有显著相关性(P<0.05)。结论间歇低氧诱发动脉粥样硬化的发生发展涉及多个基因的改变,环氧合酶2可能是重要作用基因之一。

Analysis of Gene Expression Patterns in Atherosclerosis Induced by Intermittent Hypoxia

Aim To investigate gene expression patterns of atherosclerosis (As) induced by intermittent hypoxia. Methods The total RNA were extracted from the aorta of rabbits and then reverse transcribed to make the probe. The probes hybridisate the gene chip from Afymetrix's company, the result signals were scanned by ScanArray HS-038 and analyzed by Microarray Suite Version 5.0. Results There was total 187 genes down-regulated or up-regulated, classified by cell signal transduction, immunology inflammatory response, extracellular matrix degrade, cell apoptosis, smooth muscle cell related, oncogene or tumor suppressor and cell migration. The expression level of cyclooxygenase-2 (COX-2) mRNA in abdominal aorta was significantly associated with the change of abdominal aorta endarteropathy (P<0.05) . Conclusion The process of As induced by intermittent hypoxia is correlated to many genes, gene chip, the high-flux detecting technique can provide the new ideas for the mechanism research of As. COX-2 plays an important role in As.