异丙酚预处理离体乳鼠脑皮质细胞对脑缺血再灌注损伤的保护作用

目的：探讨不同浓度异丙酚预处理离体乳鼠脑皮质细胞对脑缺血-再灌注损伤的保护作用。方法：取出生24 h以内的新生SD乳鼠的脑皮质细胞,体外培养至第7天,随机分为5组：A组（正常对照组）,B组（损伤组）,C1组（1 mg/L异丙酚预处理组）,C2组（3 mg/L异丙酚预处理组）和C3组（5 mg/L异丙酚预处理组）。C1、C2C3组第7天予以对应浓度的药物预处理,24 h后B、C1、C2、C3组予200μmol/L谷氨酸损伤0.5 h,所有组更换正常培养液继续培养24 h;观察神经细胞存活率（MTT法）、乳酸脱氢酶（LDH）漏出率、细胞凋亡率、HE染色后细胞形态变化。结果：异丙酚预处理各组MTT量不同程度高于损伤组,LDH漏出量和凋亡细胞百分比低于损伤组;HE染色后各预处理组细胞形态受损较损伤组轻,以C2组效果最佳。结论：异丙酚提前24 h预处理离体幼鼠脑皮质细胞对脑缺血-再灌注损伤有保护作用,其中以3 mg/L异丙酚保护效果最佳。

An Experimental Study on the Protective Effect of Propofol Pretreatment against Cerebral Injury Caused by Ischemia-reperfusion

Objective： To study the protective effect of propofol pretreatment against cerebral ischemia-reperfusion caused injury in rat primary cortical cultures.Methods： Cortical neurons of SD mouse aged no more than 24 hours were cultured for 7 days,and then were randomly divided into： control group（A）,ischemia-reperfusion group（B）,propofol pretreatment groups（C1,C2,C3）.Propofol of 1 mg/L,3 mg/L,5 mg/L was given to cells in groups C1,C2,and C3 respectively,and incubated for 24 hours.Then,200 mmol/L of glutamate were given into groups B,C1,C2,and C3.In half an hour later,culture media of all groups were replaced.The survival rates of nerve cells,efflux rates of LDH and the cell apoptosis rates of all the groups were determined.The cell shape was observed with HE dyetechnigue.Results： In propofol pretreated groups,the survival rates of nerve cells were significantly higher,and the LDH efflux rates and apoptosis rates were significantly lower than those in ischemia group;Cell destruction of propofol pretreated groups were slighter than that in ischemia group.Among propofol pretreated groups,the protective effect in group C2 was the best.Conclusions： Pretreatment of different concentrations of propofol in 24 hours before the ischemia-reperfusion can produce different protective effects to brain against the injury.Among the tested propofol concentration,3 mg/L is the best for protection.