Wet SEM: A Novel Method for Rapid Diagnosis of Brain Tumors |
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Authors: | Iris Barshack Sylvia Polak-Charcon Vered Behar Anya Vainshtein Ory Zik Efrat Ofek |
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Institution: | 1. barshack@sheba.health.gov.il;3. Quantomix Ltd., Rehovot, Israel |
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Abstract: | The authors present the application of wet SEM for histopathological assessment, a technology for imaging fully hydrated samples at atmospheric pressure in a scanning electron microscope (SEM). Both transmission and scanning electron microscopy techniques usually require long and complex sample preparation of the tissues. In marked contrast, a rapid preparation of tissues is described for evaluation by SEM imaging. The wet SEM technology successfully demonstrated both histological and ultrastructural features of several CNS tumors: Rosette formation and intracytoplasmic lumens were observed in ependymoma; numerous fibrillary processes in fibrillary astrocytoma; and focal rosette formation with no intracytoplasmic lumens in medulloblastoma. Application of this method simultaneously with frozen section may improve rapid intraoperative diagnosis of these intracranial tumors. |
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Keywords: | brain tumors ependymoma fibrillary astrocytoma medulloblastoma microscopy wet SEM |
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