八聚体结合转录因子4与蛋白激酶B1基因在卵巢浆液性肿瘤组织表达及耐药相关性研究

目的 研究八聚体结合转录因子4（OCT4）和蛋白激酶B1（AKT1）基因在卵巢浆液性肿瘤组织中的表达，探讨两者之间相关性及在化疗耐药中发挥的作用。 方法 全部病例来自中国医科大学附属盛京医院2004年1月至2010年10月。采用免疫组织化学SP法对67例卵巢浆液性腺癌（化疗耐药30例，化疗敏感37例）、10例卵巢交界性浆液性囊腺瘤、10例卵巢良性浆液性囊腺瘤和10例正常卵巢组织中OCT4和AKT1的表达情况进行检测。 结果 OCT4的阳性表达率在卵巢浆液性腺癌、卵巢交界性浆液性囊腺瘤、卵巢良性浆液性囊腺瘤和正常卵巢组织中依次降低，差异具有统计学意义（P=0.002）；在耐药组和敏感组的阳性表达率分别为83.33%和45.95%，差异有统计学意义（P=0.002）。AKT1蛋白在正常组、良性组、交界性组和恶性组的阳性表达率分别为0、40.00%、70.00%、62.69%，差异具有统计学意义（P=0.001）；在耐药组和敏感组的阳性表达率分别为76.67%和51.35%, 两组比较，差异有统计学意义（P=0.033）。OCT4蛋白与卵巢浆液性癌临床病理因素无关，AKT1蛋白与卵巢浆液性癌的临床分期呈正相关（P<0.05）。OCT4与AKT1蛋白在耐药组中呈正相关（r=0.388，P=0.034）；在敏感组无明显相关性（r=0.246，P=0.142）。

The expression of OCT4 and AKT1 in ovarian serous tumor and their correlation with drug resistance of chemotherapy.

Abstract： Objective To study the expression of OCT4 and AKT1 in ovarian serous tumor and their correlation with drug resistance of chemotherapy.Methods From Shengjing Hospital of China Medical University between January 2004 and October 2010，67 cases of ovarian serous cystadenocarcinoma were selected(30 cases resistant,37 cases sensitive)，10 cases of normal ovarian tissues，10 cases of ovarian benign cystadenoma and 10 cases of ovarian serous cystadenoma for test. The OCT4 and AKT1 were tested by immunohistochemistry. Results The positive rates of OCT4 in normal group, benign group, borderline group and malignant group were 0，40.00%,50.00% and 62.69%,the differences being significant(P=0.002); in drug resistance group and sensitive group they were 83.33%and 45.95%，the differences being significant(P=0.002). The positive rates of AKT1 in normal group, benign group, borderline group and malignant group were0,40.00%,70.00% and 62.69%,the differences being significant(P=0.001); in drug resistance group and sensitive group were they 76.67% and 51.35%，the differences being significant(P=0.033). The expression of OCT4 didn’t correlate with any clinicopathologic factors, but AKT1 correlated with clinical stage（P<0.05）. A positive correlation was found between the expression of OCT4 and AKT1 in drug resistance group（r=0.388,P=0.034） but not in sensitive group（r=0.246,P=0.142）. Conclusions OCT4 and AKT1 are probably involved in early carcinogenesis of ovarian serous cystadenocarcinoma and risk factors for drug resistance.