酪氨酸激酶受体阳性单核细胞对卵巢肿瘤生长及微血管、淋巴管生成作用研究

目的 探讨酪氨酸激酶受体阳性（Tie2+）的单核细胞(Tie2+-U937)对裸鼠移植瘤生长和微血管、淋巴管生成的作用。方法 2013年1月至2014年2月在上海交通大学医学院构建裸鼠卵巢肿瘤皮下和原位模型，转染Tie2+-U937和NC-U937，分别将转染了荧光的人卵巢癌细胞系(SKOV3ip1-luc)与Tie2+-U937或者NC-U937按1:10比例混匀，注入裸鼠皮下和卵巢包膜下，单独的SKOV3作为阴性对照组，观察裸鼠成瘤时间和肿瘤大小；免疫组化检测肿瘤微血管和淋巴管的表达。结果 Tie2+-U937能够促进裸鼠皮下和原位移植瘤生长。皮下成瘤模型：注射后第8、21天Tie2组肿瘤总荧光强度值大于其他两组(P<0.05)。裸鼠于21d处死并称其肿瘤重量，Tie2组(0.43±0.16)g，U937组(0.18±0.04)g，Control组(0.13±0.08)g，Tie2肿瘤重量较其他两组重(P<0.05)；Tie2组免疫组化CD31的表达明显大于其余两组(P<0.05)；原位模型：注射后第14、35天Tie2组肿瘤总荧光强度值大于其余两组(P<0.05)；Tie2组免疫组化CD31和LYVE-1的表达明显大于其余两组(P<0.05)。结论 Tie2+单核细胞能够促进卵巢肿瘤裸鼠皮下、原位移植瘤的生长，并且其促进肿瘤增殖的作用与微血管和淋巴管生成有关。

The effects of tie2+-U937 on proliferation,angiogenesis and lymphangiogenesis of ovarian carcinoma.

Abstract： Objective Aim to explore the effects of Tie2+-U937 cells on proliferation and its efftcts on angiogenesis and lymphangiogenesis of ovarian carcinoma in nude mice.Methods The ovarian carcinoma models were constructed between Janary 2013 and Fedruary 2014 in Shanghai Jiaotong University School of Medicine Human Tie2+-U937 cells and NC-U937 cells were cultured by transfection. Nude mice were injected with mix of human SKOV3ip1-luc cells and Tie2+-U937 cells or NC-U937 cells by 1:10 in the subcutaneous models and orthotopic models . SKOV3 cells were injected as control group. The proliferation time, tumor size, CD31 and LYVE-1 expression of ovarian carcinoma were detected.Results The results revealed that Tie2 led to an enhancement on tumor proliferation. In the subcutaneous models: Tie2 led to an enhancement on tumor proliferation, compared with U937 group and control group on the 8th day and 21st day. The tumor weights of different groups on the 21st day : Tie2 group(0.43±0.16)g, U937 group (0.18±0.04)g, control group (0.13±0.08)g(P<0.05).In the orthotopic models: Tie2 led to an enhancement on tumor proliferation, compared with U937 group and control group on the 14th day and 35th day. Tie2 group was illustrated the highest intratumoral CD31 and LYVE-1 expression level, as compared with the other groups (P<0.05). Conclusion Tie2+-U937 cells can promote ovarian carcinoma proliferation through angiogenesis and lymphangiogenesis in nude mice.