| 急性肺缺血再灌注引起肺组织表面活性物质相关蛋白A变化实验研究 |
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| 引用本文： | 王海英,倪松石,许文景.急性肺缺血再灌注引起肺组织表面活性物质相关蛋白A变化实验研究[J].南通医学院学报,2009,29(4). |
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| 作者姓名： | 王海英 倪松石 许文景 |
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| 作者单位： | 1. 南通大学附属医院呼吸内科,南通,226001
2. 江苏省苏北人民医院 |
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| 摘 要： | 目的:探讨肺急性肺缺血再灌注(ischemia reperfution,I/R)时表面活性物质相关蛋白A(surfactant-associated protein A,SP-A)含量的变化与肺损伤的关系。方法:40只健康的SD大鼠,雌雄不拘,随机分成5组:假手术组(S)、单纯缺组(I)、再灌注1h组(R1组)、再灌注2h组(R2组)、再灌注6h组(R3组)。根据Eppinger模型建立原位阻断左肺门大鼠温肺I/R模型。术毕测定各组血清丙二醛(MDA)及肺湿/干重比例(W/D)、病理观察、用Western-Blot及免疫组化法检测各组肺组织中SP-A。结果:(1)MDA、肺W/D在I组即较S组开始升高,予再灌注后,各组MDA、肺W/D随再灌注时间的延长明显升高(P<0.05);(2)SP-A在S组表达最多,在I组表达减少,再灌注后随时间的延长表达更少(P<0.05)。结论:大鼠肺急性I/R后,肺表面活性物质相关蛋白A减少是造成肺一系列损害的重要因素。
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| 关 键 词： | 缺血再灌注 丙二醛 肺湿/干重 肺表面活性物质相关蛋白A |
The experimental research of the change of surfactant-associated protein A resulted from the acute lung ischemia-reperfusion |
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| WANG Haiying,NI Songshi,XU Wenjin.The experimental research of the change of surfactant-associated protein A resulted from the acute lung ischemia-reperfusion[J].ACTA Academiae Medicinae Nantong,2009,29(4). |
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| Authors: | WANG Haiying NI Songshi XU Wenjin |
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| Institution: | 1 Department of Respiratory Medicine; the Affiliated Hospital of Nantong University; Nantong 226001; 2Subei Perple's Hospital of Jiangsu Province); |
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| Abstract: | Objective:To investigate the change of SP-A and the relationship between SP-A's alternation and the injury,the quantity of MDA in serum,the W/D and the quantity of SP-A in the lung tissue were detected. Methods:Without considering their sex,40 healthy SD rats were chosen and divided into five groups,8 rats in every group. They were the rats who received a left anterolateral thoracotomy without clamping the left hilum as Sham group (S),the rats whose left hilums were clamped for 1 hour without reperfusion as... |
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| Keywords: | Ischemia-reperfusion Minimum descent altitude Surfactant-associated protein A |
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