mTOR和VEGF在胃癌中的表达及与预后关系的研究

目的：观察mTOR和VEGF在胃癌中的表达，探讨它们在胃癌发生发展中的意义和对胃癌患者预后的影响。方法：采用组织芯片和免疫组化检测mTOR和VEGF在1072例胃癌中的表达。结果：mTOR和VEGF在胃癌中总表达率分别为50．8％和59．4％。mTOR过度表达与肿瘤部位（贲门、胃底）、腺癌、高／中分化、T1／T2、Ⅰ／Ⅱ期以及PCNA表达有关；VEGF过度表达与年龄（〉60岁）、男性、肿瘤部位（贲门、胃底）、腺癌、高／中分化、肿瘤浸润（T3～T4）、淋巴结转移、PCNA表达以及p53表达有关。mTOR和VEGF共表达率为39．6％。Cox模型结果显示，mTOR和VEGF是胃癌独立的预后因素。结论：mTOR／VEGF信号通路在胃癌中高度激活，mTOR和VEGF的表达是胃癌预后的重要影响因素，可作为胃癌治疗的靶点。

Expression of mTOR and VEGF in gastric carcinoma and its correlation with prognosis

Objective：To detect the relationship between the expression of mTOR and VEGF in gastric carcinoma and to investigate its influence on prognosis. Methods：Tissue microarrays containing 1 072 cases of tumor specimens were constructed, mTOR and VEGF expression were investigated by immunohistochemical studies. Results： The overall expression rates of mTOR and VEGF in cancer were 50. 8% and 59.4% , respectively. Over expression of mTOR protein was associated with many complex factors such as tumor location（ upper gastric ）, adenocarcinoma, high/moderate differentiation, T1/T2, Ⅰ /Ⅱ stage, and PCNA expression. VEGF overexpression was closely related to age （ 〉 60y）, male patients, tumor location （ upper gastric）, adenocarcinoma, high/moderate differentiation, gastric wall invasion（T3-T4）, LN metastases, PCNA expression and p53 expression. The co-expression rates of mTOR and VEGF was 39. 6%. According to the Cox proportional hazards model, mTOR and VEGF expression, were correlated with progno- sis. Conclusion： mTOR/VEGF signaling is frequently activated in human gastric cancer, mTOR, VEGF and combination of mTOR and VEGF play a very important role in prognosis. They may be regarded as the potential molecular targets for therapy.