结肠癌组织肿瘤相关巨噬细胞VEGF-C/D的表达及与淋巴结转移的关系

目的 了解结肠癌肿瘤相关巨噬细胞中VEGF-C/D的表达,探讨其对结肠癌淋巴结转移的影响及可能机制.方法 采用特异的巨噬细胞标记物CD68等抗体以免疫组化染色的方法,并用双染、连续切片对比等对45例结肠癌标本中巨噬细胞VEGF-C/D的表达进行检测,同时行巨噬细胞计数,并统计分析两者间存在的关系.结果 在结肠癌相关巨噬细胞中检测到两种淋巴管生成因子的表达.统计分析显示VEGF-C表达与巨噬细胞计数具有相关性.有淋巴结转移者VEGF-C阳性细胞率高于无转移组.结论 结肠癌肿瘤组织中,不仅肿瘤细胞可分泌表达VEGF-C/D,肿瘤相关巨噬细胞也可表达VEGF-C/D.肿瘤相关巨噬细胞可通过诱导结肠癌局部淋巴管生成,对肿瘤的转移可起到一定的作用.

Correlation between VEGF-C/D expressions in tumor associated macrophages and lymph node metastasis in colon cancer

Objective To discuss the correlation between the VEGF-C/D expressions in tumor associated macrophage (TAM) and lymph node metastasis in colon cancer. Methods Forty-five colon cancer samples proven pathologically to be adenocarcinoma were stained by immunohistochemical method for VEGF-C/D and CD68. The correlation of VEGF-C/D expressions, macrophage counting and lymph node metastasis were analyzed statistically. Results The expressions of VEGF-C/D were detected in TAMs. VEGF-C expression was obviously higher in the samples with lymphatic metastasis. VEGF-C expression in colon cancer was related to the number of TAMs. Conclusion TAMs are important in the lymph node metastasis, because they can express VEGF-C/D and affect the lymphangiogenesis of colon cancer.