瘤内缓释治疗对B16黑色素瘤小鼠影响的实验研究

目的：探讨肿瘤内缓释治疗对B16黑色素瘤的影响及其机制。方法:将皮下接种B16肿瘤细胞后的成瘤小鼠随机分成4组进行治疗：①生理盐水（NS）对照组，瘤内注射NS；②阿糖胞苷（Ara-C）对照组，瘤内注射Ara-C；③单纯缓释治疗组，瘤内注射Ara-C+缓释液；④免疫缓释液治疗组，瘤内注射Ara-C+缓释液+二硝基苯 (DNP)。4d后重复治疗，观察各组小鼠肿瘤的生长情况。两次治疗后1周分离瘤体，HE及网状纤维、弹力纤维、胶原纤维染色，观察瘤体病理改变，用流式细胞术检测脾内CD4⁺T细胞、CD8⁺T细胞浸润情况。结果:缓释治疗能够控制肿瘤的生长；HE染色显示，两缓释治疗组瘤内出现大片坏死区域，纤维特殊染色显示，两缓释治疗组三种纤维含量均明显增加(P＜0.01)；流式细胞术检测表明，缓释治疗组的CD4+T细胞数量明显增多(P＜0.05)，但CD4⁺/CD8⁺T比值无明显变化。结论:瘤内缓释治疗通过直接破坏肿瘤细胞及增加肿瘤抗原性，从而发挥明显的抗肿瘤作用，为肿瘤治疗提供了新的思路。

Slow intra-tumor release of drugs on B16 melonoma in mice

Objective To explore the effects and mechanism of slow intra-tumor release of drugs on B16 melonoma in mice.Methods Tumor-bearing mice were randomly divided into four groups: the normal saline group,the Ara-C group,the AraC plus releasing drugs group and the Ara-C plus releasing drugs and DNP group.Four days later,mice were treated again and the gross tumor volume was determined.Then 1 week later,the contents of three fibers were detected in the interstitial substances of the tumor and the infiltrations of T cells were determined in the splenic organs.Results HE staining results showed that there was a large part of stenosis in the tumor,the fibers in the matrix of tumor were significantly increased and the CD4 T cells were also significantly increased in the two slow-release groups.Conclusion Slow-release of drugs into the tumor is one of the effective managements for directly inhibiting tumor growth and preventing micro-metastasis.