| 荧光染色发光二极管显微镜检测结核分枝杆菌的效果分析 |
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| 引用本文： | 陈燕梅,钱明,江勇,陈涛,李海成,钟球.荧光染色发光二极管显微镜检测结核分枝杆菌的效果分析[J].中国防痨通讯,2011,33(9):585-587. |
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| 作者姓名： | 陈燕梅 钱明 江勇 陈涛 李海成 钟球 |
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| 作者单位： | 广东省结核病防治研究所参比实验室 |
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| 基金项目： | 广东省医学科研课题项目，科技重大专项 |
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| 摘 要： | 目的： 探讨荧光染色发光二极管（LED）显微镜镜检对结核分枝杆菌的检出效果。方法： 收集232例可疑结核病患者的痰标本,每位患者收集3份痰（即时痰、清晨痰和夜间痰），共696份痰标本。分为萋-尼染色(Z-N)法镜检组（简称Z-N组），荧光染色发光二极管显微镜镜检组（简称LED组）。对每份痰标本同时采用上述两种镜检法进行检测。比较两者的检查结果,分析两种方法检查结核分枝杆菌的阳性率差异情况。结果： LED组检出率为37.9%（88/232），Z-N组检出率为26.7%（62/232），经检验, χ2=6.66,P＜0.01,差异有统计学意义。结论： LED显微镜镜检具有阳性检出率高等优点。
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| 关 键 词： | 分枝杆菌 结核 显微镜检查 荧光 染色与标记 |
| 收稿时间： | 2011-07-04 |
Efficiency of fluorescence microscopy with light emitting diodes in detecting Mycobacterium tuberculosis |
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| CHEN Yan-mei,QIAN Ming,JIANG Yong,CHEN Tao,LI Hai-cheng,ZHONG Qiu.Efficiency of fluorescence microscopy with light emitting diodes in detecting Mycobacterium tuberculosis[J].The Journal of The Chinese Antituberculosis Association,2011,33(9):585-587. |
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| Authors: | CHEN Yan-mei QIAN Ming JIANG Yong CHEN Tao LI Hai-cheng ZHONG Qiu |
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| Institution: | Reference Laboratory Anti-tuberculosis Institute of Guangdong Province |
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| Abstract: | Objective To evaluate the efficiency of fluorescence microscopy with light emitting diodes（LED-FM） in screening Mycobacterium tuberculosis. Methods Sputum samples of 232 suspected patients were collected and there were totally 696 samples including 3 samples from each patient.All samples were detected by Ziehl-Neelsen（Z-N） stain microscopy and LED-FM respectively. Results The positive rates of LED-FM and Z-N were 37.9%（88/232） and 26.7%（62/232） with significant difference（χ^2=6.66,P〈0.01）. Conclusion LED-FM in detecting Mycobacterium tuberculosis is superior to the conventional method. |
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| Keywords: | Mycobacterium tuberculosis Microscopy fluorescence Staining and labeling |
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