颗粒显色指示技术快速检测结核分枝杆菌对吡嗪酰胺耐药性的价值

目的 探讨颗粒显色指示技术快速测定结核分枝杆菌（Mycobacterium tuberculosis，Mtb）对吡嗪酰胺耐药性的临床应用价值。方法 应用颗粒显色指示法测定102株Mtb对吡嗪酰胺耐药性，并与美国BD公司BACTEC MGIT-960分枝杆菌检测系统结果进行比较。 结果 颗粒显色指示法测定102株Mtb临床分离株，结果对吡嗪酰胺敏感70株、耐药32株，BACTEC MGIT-960法测定结果敏感69株、耐药33株；两法测定均敏感67株、均耐药30株。如以BACTEC MGIT-960法药敏结果为判断标准，则颗粒显色指示法测定吡嗪酰胺耐药性的敏感度为90.9%(30/33)，特异度为97.1%（67/69)，阳性预测值为93.8%(30/32)，阴性预测值为95.7%(67/70)，准确性为95.1%(97/102)。结论 颗粒显色指示技术快速测定Mtb吡嗪酰胺耐药性简便快速，操作不需特殊仪器设备，具有很高的临床应用价值。

The value of particle colorimetric technique in rapid detection of Mycobacterium tuberculosis resistance to pyrazinamide

Objective To explore the clinical application value of particle colorimetric technique in rapid detection of Mycobacterium tuberculosis(Mtb) resistance to pyrazinamide(PZA). Methods 102 strains of Mtb resistance to PZA was measured by particle colorimetric technique,and was compared with the results of BACTEC MGIT-960 Mycobacterium detection system by American BD Corporation. Results Through particle colorimetric technique,in 102 strains of Mtb,70 of them were PZA sensitive,32 were PZA resistant.While under...