亚胺培南耐药铜绿假单胞菌氨基糖苷类修饰酶基因型研究

目的了解中国5个地区亚胺培南耐药的铜绿假单胞菌部分氨基糖苷类修饰酶分布情况。方法从成都、杭州、北京、上海和广州收集对亚胺培南耐药的铜绿假单胞菌,使用PCR法扩增16种氨基糖苷类修饰酶基因。结果共收集了146株亚胺培南耐药的铜绿假单胞菌。16种氨基糖苷类修饰酶基因中有6种基因型获得阳性的PCR结果,氨基糖苷类修饰酶基因总检出率为65.06%,各种氨基糖苷类修饰酶基因检出率分别为aac（3）-Ⅱ33.6%、aac（6＇）-Ⅰ15.8%、aac（6＇）-Ⅱ19.9%、ant（2″）-Ⅰ28.8%、ant（3″）-Ⅰ14.4%和aph（3＇）-Ⅵ4.8%。氨基糖苷类修饰酶基因的分布存在地区差异,其中杭州和上海6种AMEs基因均被检测出,成都和北京检测出4种AMEs基因,广州的IRPA中仅检测出3种AMEs基因。结论亚胺培南耐药的铜绿假单胞菌氨基糖苷类修饰酶基因携带率高;氨基糖苷类修饰酶基因型在中国5个地区间分布存在差异。

Genotypes of Aminoglycosides Modifying Enzymes in Imipenem-Resistant Pseudomonas Aeruginosa

Objective To describe and compare the distributi ons of aminogl ycosides modifying enzymes(AMEs) in imipenem-resistant Pseudomonas aeruginosa(IRPA) collected from 5 cities in China.Methods A total of 14 6 strains of IRPA were collected from 5 cities of China(Chengdu,Hangzhou,Beijing,Shanghai,an d Guangzhou).The polymerase chain reaction(PCR) were used to amplify the gen es of AMEs in IRPA.Results Six positive genotypes were amplifi ed out of 16 geno types of AMEs by PCR.The total positive rate of AMEs is 65.06%.The positive rate s of genes of aac(3)-Ⅱ,aac(6′)-Ⅰ,aac(6′)-Ⅱ,ant(2″)-Ⅰ,ant(3″)-Ⅰ and aph(3′)-Ⅵ were 33.6%,15.8%,19.9%,28.8%,14.4%,an d 4.8%,respecti ve ly.The genotypes of AMEs were discrepant in different areas as 6 genotypes in Hu angzhou and Shanghai,4 genotypes in Chengdu and Beijing,and 3 genotypes in Gua ngzhou.Conclusion The results show that the positive rate of A MEs genes is high in IRPA,and the distribution is discrepant among different areas.