| 普通轮状病毒Wa的空斑实验 |
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| 引用本文： | 于修平,温乐英.普通轮状病毒Wa的空斑实验[J].山东大学学报(医学版),1986(4). |
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| 作者姓名： | 于修平 温乐英 |
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| 作者单位： | 山东医科大学微生物学教研室
(于修平)，山东医科大学微生物学教研室(温乐英) |
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| 摘 要： | 用胰酶预先处理轮状病毒,琼脂层中加入二异氨乙基葡聚糖时,Wa能在MA_(104)细胞上形成空斑。与猴的轮状病毒SA_(11)相比,两者空斑的形态和大小相似,但Wa形成空斑要求条件较高,且空斑出现较迟。
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| 关 键 词： | 轮状病毒 空斑测定 琼脂 胰蛋白酶 |
A PLAQUE ASSAY FOR THE ORDINARY ROTAVIRUS WA |
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| Yu Xiuping Dept. of Microbiology,Shandong Medical University.A PLAQUE ASSAY FOR THE ORDINARY ROTAVIRUS WA[J].Journal of Shandong University:Health Sciences,1986(4). |
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| Authors: | Yu Xiuping Dept of Microbiology Shandong Medical University |
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| Abstract: | A quantitative and reproducible plaque assay for the measurementof ordinary rotavirus Wa is described. When these is trypsin and diethylamino-ethyl-dextran in the agar overlay, Wa can produce plaque in MA_(104) cell line.In comparing the ordinary rotavirus Wa with simian rotavirus SA_(11), theirplaque size and morphology are similar, but plaque formation of Wa is slower,and its required condition is more strict. In order to observe the plaque of Wa,two layers of agar should be overlaid. The secondary layer agar contains neutralred. The plaques are counted at the 7th day |
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| Keywords: | Rotaviruses Plaque assay Agar Trypsin |
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