| ELISA测定德国小蠊卵黄蛋白含量方法的建立 |
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| 引用本文： | 于长明,李成文,刘泉. ELISA测定德国小蠊卵黄蛋白含量方法的建立[J]. 寄生虫与医学昆虫学报, 1998, 0(4) |
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| 作者姓名： | 于长明 李成文 刘泉 |
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| 作者单位： | 军事医学科学院微生物流行病研究所 |
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| 摘 要： | 本文采用蒸馏水沉淀法提取了德国小蠊卵黄蛋白，免疫家兔，并将所得抗血清经雄虫血淋巴吸收，获得抗卵黄蛋白特异抗体，用ＤＥＡＥ－纤维素批次法纯化抗体ＩｇＧ，并用辣根过氧化物酶标记，建立ＥＬＩＳＡ直接法测定卵黄蛋白含量的方法。
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| 关 键 词： | 德国小蠊 卵黄蛋白 酶标抗体 ELISA |
DEVELOPMENT OF AN ENZYME LINKED IMMUNOSORBENT ASSAY (ELISA) FOR MEASUREMENT OF VITELLIN CONTENT IN BLATTELLA GERMANICA |
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| Yu Changming Li Chengwen Liu Quan. DEVELOPMENT OF AN ENZYME LINKED IMMUNOSORBENT ASSAY (ELISA) FOR MEASUREMENT OF VITELLIN CONTENT IN BLATTELLA GERMANICA[J]. Acta Parasitologica et Medica Entomologica Sinica, 1998, 0(4) |
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| Authors: | Yu Changming Li Chengwen Liu Quan |
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| Abstract: | The present paper is the result of development of an ELISA for measurement of vitellin content in Blattella germanica, the vitellin of Blattella germanica was extraction with 0 4M NaCl and subsequent precipitation in cold distilled water. A ployclonal antibody against vitellin was prepared in male rabbits and made the specific antibody for vitellin by absorption with male cockroach haemolymph. The ELISA was established to quantify vitellogenin of the insect through purifying and conjugating the IgG to HRP. |
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| Keywords: | Vitellin ELISA Blattella germanica Enzyme labelled antibody |
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