Aurora A 在人前列腺癌中的表达

目的观察Aurora A在人前列腺癌中的表达情况,探讨Aurora A是否在前列腺癌发生发展中起一定作用。方法采用RT-PCR、免疫组织化学和Western blot,检测在前列腺癌组织及其细胞系中Aurora A mRNA和蛋白表达。同时,在前列腺癌细胞系PC3中导入靶向Aurora A mRNA的DNA核酶,观察抑制Aurora A对前列腺癌细胞生长的影响。结果91%的前列腺癌组织和三种前列腺癌细胞系(PC3、LNCaP和Du145)中Aurora A表达阳性,而且,靶向Aurora A mRNA的DNA核酶导入PC3细胞后抑制了Aurora A表达,使PC3细胞停滞在G2/M期,并促进细胞凋亡。结论Aurora A可能在前列腺癌形成中起一定作用,Aurora A可望成为前列腺癌治疗中有价值的靶点。

Effect of Aurora A on Carcinogenesis of Human Prostate Cancer

Objective To investigate whether Aurora A is involved in prostate cancer carcinogenesis Methods The expressions of Aurora A mRNA and protein in prostate cancer tissue and cell line were measured using RT-PCR,immunohistochemistry and Western blot analyses.Meanwhile,A DNAzyme targeting at Aurora A mRNA was performed to detect the inhibition effect of Aurora A in regulating the growth of prostate cancer cell line PC3.Results Aurora A expression was up-regulated in 91% prostate cancer tissue as well as in prostate cancer cell lines such as PC3,LNCaP and Du145.Furthermore,Aurora A expression suppressed by cell transduction of a DNAzyme targeting at Aurora A mRNA made the PC3 cells both arrest the cell cycle in G2/M phase and increase the cell apoptosis.Conclusions These findings suggest that Aurora A up-regulation may be involved in carcinogenesis of prostate cancer,and that aurora A gene may be a valuable target for treatment of prostate cancer.