The Effects of Concentrated Eluted Anti-Red Blood Cell Antibodies on the in Vivo Survival of Normal Red Blood Cells

1. A method has been described for the preparation and sterilization of aconcentrated eluate from human red cell stroma.

2. Red cells sensitized by such an eluate prepared from normal controlred cells showed entirely normal in vivo survival, as did cells sensitized byeluate from anti-H coated cells.

3. Sensitization of red cells by concentrated eluates from a patient withCoombs-negative acquired hemolytic anemia and from a patient with Coombs-positive acquired hemolytic anemia did not cause significant alteration in thein vivo survival of the red cells.

4. Red cells sensitized by the concentrated eluate from anti-D sensitizedcells disappeared from the recipient’s circulation very rapidly and were sequestered in the spleen, indicating preservation of the physiologic propertiesof the antibody throughout the elution, concentration and sterilization procedures.

Submitted on June 22, 1959 Accepted on September 27, 1959