牛磺酸对离体视网膜组织脂质过氧化反应保护机制的实验研究

目的：探时牛磺酸对离体家免视网膜组织脂质过氧化反应、超氧化物歧化酶(superoxide dismutase,SOD)及谷胱过氧化物酶(glutathione，peroxidase,GSH-Px)活性的影响。 方法：将家兔眼球视杯置于4组(对照组、模型组、牛磷酸组、β—胡萝卜素组)培养液中，5%CO₂，37°C温育．分别于24小时和48小时时后测定视网膜组织中SOD，GSH-Px、蛋白及丙二醛(malondialdehyde，MDA)．结果：牛磺酸与β-胡萝卜索一样可以抑制视网膜组织内脂质过氧化反应，减少MDA生成；对SOD保护作用不确定，对GSH-Px无保护作用。 结论；牛磺酸可抑制视网膜组织脂质过氧化反应，其机制可能与对SOD,GSH—Px活性的膨响无关。 （中华眼底病杂志，1996，12：183-185）

THE PROTECTIVE MECHANISM OF TAURINE IN LIPID PEROXIDATION OFRABBIT RETINA IN VITRO

PURPOSE: To probe the effect of taurine on lipid peroxidation,surperoxide dismutase(SOD) and glutathione peroxidase(GSH-Px)in retina in vitro. METHODS: The animal eye cups were put into media(divided into four groups:control,model,taurine and β-carotene) respectively,and incubated at 37°C in a humidified atmosphere of 5% CO₂/95% air. After 24h or 48h ,the retinas were taken out from the media and the SOD,GSH-Px ,protein and malondialdehyde(MDA) were examined. RESULTS:Taurine could inhibite lipid peroxidation in retina ,decrease MDA level ,could not protect GSH-Px activity in retina. The effect of taurine on SOD activity in retina was also uncertain.CONCLUSION:Taurine can inhibit lipid peroxidation in retina in vitro,but the mechanism of thathas nothing to do with the effect of taurine on SOD activity and GSH-Px activity. （Chin J Ocul Fundus Dis,1996,12: 183-185 ）