三磷酸腺苷对小鼠肌源性干细胞增殖和细胞周期的影响

目的 探讨三磷酸腺苷（ATP）对体外培养的小鼠肌源性干细胞（MDSC）增殖和细胞周期的影响。方法 根据细胞贴壁能力的差异。采用差速贴壁法分离培养MDSC，应用MTT比色测定法和流式细胞仪（FCM）分析检测实验组和对照组细胞的增殖情况和细胞周期的变化。结果 经ATP作用后。A490波长的光吸收值明显升高，处于S期的MDSC数量（S％）明显升高，G1％降低，增殖指数PrI值（S＋G2M）％增高。结论 ATP能促进小鼠MDSC增殖。这一作用可能是通过促进MDSC从G1期进入S期来实现的。

Effects of ATP on the proliferation and cell cycle of mouse muscle-derived stem cells

Objective To evaluate the effects of ATP on the proliferation and cell cycle of mouse muscle-derived stem cells (MDSC).Methods On the basis of the difference of cellular adherent ability,MDSC from primary cultures of mouse skeletal muscle were isolated and purified by the preplate technique.MTT colorimetric assay and flow cytometry (FCM) were used to detect the effects of cell proliferation and the change of cell cycle.Results In experimental group,the photoabsortion values in A_ 490 was increased significantly.FCM revealed that the number of MDSC in S phase (S%) was significantly increased,G_1% obviously decreased and the PrI (S+G_2M)% enhanced when the cells were stimulated with ATP.Conclusion ATP enhances the proliferation of MDSC,which may be accomplished by accelerating the transition from G_1 to S.