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一些保肝药物对原代培养大鼠肝细胞糖原合成功能的影响
引用本文:王惠芬,丛铮. 一些保肝药物对原代培养大鼠肝细胞糖原合成功能的影响[J]. 药学学报, 1989, 24(9): 653-658
作者姓名:王惠芬  丛铮
作者单位:北京医科大学药理教研室,北京医科大学药理教研室 北京 100083,北京 100083
摘    要:本文参照PO Seglen的方法并加以修改,建立了原代培养大鼠肝细胞糖原合成功能的测定体系。观察到联苯双酯既能使正常肝细胞合成糖原增加88%,又能保护肝细胞完全拮抗四氯化碳对其功能的损伤;银耳多糖能使四氯化碳对肝细胞糖原合成功能的损伤减轻57%;去甲斑蝥素10μg/ml能增加肝细胞糖原合成,浓度增加到100μg/ml时,此作用减弱,1000μg/ml则明显抑制糖原的合成,而且在10~100μg/ml浓度时,即能加强四氯化碳的损伤作用;100μg/ml CL1500和熊果酸二钠单独应用可增加肝细胞糖原合成,但与四氯化碳同时应用,反而加重对糖原合成的抑制作用。

关 键 词:糖原合成  四氯化碳  联苯双酯  银耳多糖
收稿时间:1988-10-10

INFLUENCE OF SEVERAL CHINESE DRUGS ON THE GLYCOGEN SYNTHESIZING FUNCTION OF PRIMARY CULTURED ADULT RAT HEPATOCYTES
HF Wang and Z Cong. INFLUENCE OF SEVERAL CHINESE DRUGS ON THE GLYCOGEN SYNTHESIZING FUNCTION OF PRIMARY CULTURED ADULT RAT HEPATOCYTES[J]. Acta pharmaceutica Sinica, 1989, 24(9): 653-658
Authors:HF Wang and Z Cong
Abstract:The influence of several Chinese drugs on the glycogen synthesizing function of normal and carbon tetrachloride-injured hepatocytes was investigated. Hepatocytes prepared from rats fasted for 16 h were incubated with 50 mmol/L glucose. Glycogen content was determined 30 and 90 min after incubation with normal and CCl4-injured cells, respectively. Insulin was used as positive control which increased glycogen content and the data coincided with that in the literature. The following results were obtained (1) Biphenyl-dimethyl-dicarboxylate (BDD) in 100 micrograms/ml concentration increased glycogen content of normal hepatocytes by 88%. It protected cells against CCl4-injury:BDD 10 micrograms/ml remarkably decreased CCl4-induced reduction of glycogen and 100 micrograms/ml showed complete protection. (2) Tremella poly-saccharide slightly increased glycogen content in normal cells, but in a concentration of 100 micrograms/ml it decreased CCl4-induced reduction of glycogen significantly. (3) Low concentration of norcatharidin (10 micrograms/ml) increased glycogen content of normal cells, but at 100 micrograms/ml concentration this effect disappeared. Furthermore, it intensified the toxic effect of CCl4 on glycogen at 10-100 micrograms/ml. (4) CL1500 (100 micrograms/ml) increased glycogen content of normal cells, but it intensified CCl4-injury effect on glycogen at the same concentration.
Keywords:Glycogen synthesis  Carbon tetrachloride  Biphenyl-dimethyldicarboxylate  Tremella polysaccharide
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