| 杂色曲霉素对体外小鼠腹腔巨噬细胞白细胞介素12表达与分泌的影响 |
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| 引用本文： | 邢凌霄,张祥宏,尹桂然,李月红,王俊灵,严霞,王凤荣.杂色曲霉素对体外小鼠腹腔巨噬细胞白细胞介素12表达与分泌的影响[J].中国药理学与毒理学杂志,2005,19(3):205-208. |
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| 作者姓名： | 邢凌霄 张祥宏 尹桂然 李月红 王俊灵 严霞 王凤荣 |
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| 作者单位： | 1. 河北医科大学基础所病理室,河北,石家庄,050017
2. 河北医科大学第二医院妇产科研究室,河北,石家庄,050000 |
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| 基金项目： | 国家科技部基础研究重大项目前期研究专项资助项目 (2 0 0 1CCC0 0 5 0 0 )，河北省自然科学基金重点资助项目 (30 135 0 )~~ |
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| 摘 要： | 目的 探讨杂色曲霉素(ST)对巨噬细胞免疫功能的影响。方法 分别采用半定量逆转录聚合酶链反应及酶联免疫吸附法,研究0 .12 5 ,0 .2 5 ,0 .5 ,1.0和2 .0mg·L- 1ST预处理对小鼠腹腔巨噬细胞白细胞介素(IL) 12mRNA表达及其蛋白分泌的影响。结果 在0 .12 5～1.0mg·L- 1范围内,ST处理对小鼠腹腔巨噬细胞IL 12p4 0和IL 12p35mRNA的表达均有一定的抑制作用。但在蛋白水平,对IL 12分泌的影响不明显。当ST浓度达到2 .0mg·L- 1时,可明显抑制IL 12p4 0mRNA的表达及IL 12的分泌。结论 ST可抑制巨噬细胞IL 12的表达或分泌,提示ST可能在一定程度上对机体的细胞免疫功能产生不利影响。
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| 关 键 词： | 杂色曲霉素 白细胞介素12 巨噬细胞 腹膜 逆转录聚合酶链反应 |
| 收稿时间： | 2004-8-30 |
Effect of sterigmatocystin on interleukin-12 expression and secretion of murine peritoneal macrophage cells in vitro |
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| XING Ling-xiao,ZHANG Xiang-Hong,YIN Gui-Ran,LI Yue-hong,WANG JUN-LING,YAN Xia,WANG Feng-Rong.Effect of sterigmatocystin on interleukin-12 expression and secretion of murine peritoneal macrophage cells in vitro[J].Chinese Journal of Pharmacology and Toxicology,2005,19(3):205-208. |
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| Authors: | XING Ling-xiao ZHANG Xiang-Hong YIN Gui-Ran LI Yue-hong WANG JUN-LING YAN Xia WANG Feng-Rong |
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| Institution: | (1. Department of Experimental Pathology, Hebei Medical University, Shijiazhua ng 050017, China; 2. Department of Gynecology and Obstetrics, the Second Hospi tal, Hebei Medical University, Shijiazhuang 050000, China) |
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| Abstract: | AIM To explore the effect of sterigmatocystin (ST) on immunologic function of macrophages. METHODS The secretion and expression of interleukin(IL)- 12 of murine peritoneal macrophages after ST pretreatment at different concentrations（0.125，0.25，0.5，1.0 and 2.0 mg·L-1） were studied with ELISA and semi-quantitative RT-PCR method, respectively. RESULTS In the concentration range 0.125－1.0 mg·L-1, ST could inhibit the expression of IL-12 p40 and IL-12 p35 at mRNA level, but no effect on the secretion of IL-12 protein was found. ST at 2.0 mg·L-1 could significantly inhibit the expression of IL-12 p40 mRNA and the secretion of IL-12 protein. CONCLUSION ST could inhibit the expression or secretion of IL-12 of murine peritoneal macrophages. |
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