4NQO诱发A／J系小白鼠肺腺癌的研究

目的：通过A／J系小鼠肺腺癌模型，探讨肺肿瘤细胞的起源和恶性转化过程。方法：应用4－氮喹啉1－氧化物（4QO）化学物质为促癌剂，建立A／J系列小鼠肺癌的模型。观察诱发肿瘤时间、肿瘤数目及与类型的关系。结果：RNQO诱发5个月时，共发现肺肿瘤17处，其中肺泡上皮增生（AP）6处。肺泡腺瘤（AA）9处，乳头状腺瘤（PA）2处；诱发7个月时共发现肺肿瘤19处，AP为7处，AA为11处，PA为1处，诱发9个月时共发现肺肿瘤27处，AP为5处，AA为17处，PA为3处，发现乳头状腺癌（PC）2处。结论：4NQO能诱发A／J系小鼠肺腺癌。诱发肿瘤的时间长短与发生肿瘤的组织学类型有密切关系。

Induction of Lung Adenocarcinoma in A/J Mouse by 4-Nitroquinoline 1-Oxide

Objective: With the formation of the model of adenocarcinoma of lung in A/J mouse induced by 4 nitroquinoline 1 oxide(4NQO), we studied the development of lung adenocarcinoma and its malignant transformation.Methods: The 4NQO was used as carcinogen to form the model of adenocarcinoma of lung in A/J mouse. We observed the time of tumor induction, number of trmors, and the relationship between the number of tumors and the histologic types.Results: At the 5th month of using 4NQO, there were 17 focuses of carcinoma in lung , including 6 of alveolar proliferation (AP), 9 of alveolar adenomas (AA), and 2 of papillary adenomas (PA). At the 7th month, 19 focuses of carcinoma in lung had been found, including 7 of AP, 11 of AA,and 1 of PA. At the 9th month, 27 focuses had been found, 5 of AP, 17 of AA, 3 of PA, and 2 of papillary carcinomas.Conclusion: The 4NQO could induce the lung adenocarcinoma in A/J mouse. The induction time was closely related with the histological type of the neoplasm.