Serum miRNA profiling identifies miR-150/30a as potential biomarker for
workers with damaged nerve fibers from carbon disulfide |
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Authors: | Li GUO Chen LUO Jingjing FAN Zhiguo HOU Xiaoming JI Feng CHEN Baoli ZHU Chunhui NI |
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Institution: | 1.Department of Epidemiology and Biostatistics, School of Public Health, Nanjing Medical University, China;2.Department of Occupational Medicine and Environmental Health, School of Public Health, Nanjing Medical University, China;3.Center for Disease Control and Prevention of Jiangsu Province, China |
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Abstract: | As crucial small regulatory molecules, serum microRNAs (miRNAs) have been widely
identified as potential noninvasive biomarkers. To survey and identify serum miRNAs
associated with workers who had experienced injury to their nerve system from carbon
disulfide (CS2), we profiled abnormally expressed miRNAs using the microarray
technique and further performed qRT-PCR validation in case and control samples (n=20).
Microarray profiling in pooled RNA samples showed that many miRNAs in workers exposed to
CS2 were aberrantly expressed. Based on control samples exposed to
CS2, a great amount of abnormal miRNAs, including some miRNA gene clusters
and families, were obtained from microarray datasets. Most of deregulated miRNAs were
up-regulated, and almost all miRNAs showed consistent expression patterns between workers
with different numbers of damaged nerve fibers. Functional enrichment analysis suggested
that these abnormal miRNAs showed versatile roles by contributing to multiple biological
processes. Some aberrantly expressed miRNAs were characterized as miRNA gene clusters or
families, and they always showed consistent expression patterns. miR-150 and miR-30a were
selected to be further validated by qRT-PCR as up-regulated species, and they could
discern case samples from control samples. miR-150 and miR-30a may be potential
noninvasive biomarkers for a damaged nervous system. |
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Keywords: | microRNA (miRNA) Carbon disulfide (CS2) Biomarker |
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