急性B淋巴细胞白血病CD304表达的临床意义

目的：探讨急性B淋巴细胞白血病(B-ALL)CD304表达的临床意义及其应用价值。方法：采用流式细胞术(FCM)检测初诊的32例B-ALL细胞CD304和相关白血病抗原表达、5例急性T淋巴细胞白血病(T-ALL)和10例良性血液病对照者细胞CD304的表达；21份初诊时融合基因和CD304共阳性的治疗后B-ALL样本，FCM和PCR检测微小残留病(MRD)水平，比较两者符合率。结果：BCR-ABL1阳性病人CD304表达阳性率高于E2A-PBX1和融合基因阴性病人(P<0.05)。5例T-ALL细胞和10例对照者B细胞上CD304均阴性表达，初诊43.75%(14/32)B-ALL病人CD304阳性表达：10例BCR-ABL阳性(12例)、1例TEL-AML1阳性和3例融合基因阴性(14例)病人CD304阳性表达，而3例E2A-PBX1阳性和2例MLL/AF4阳性病人CD304阴性表达。CD304阳性病人B-ALL细胞CD66c表达频率升高(P<0.01)。21例MRD中，PCR阳性11份，FCM阳性10份，共阳性10份，与PCR结果比较，FCM阳性符合率为91%,总符合率...

The clinical significance of expression of CD304 in B-cell acute lymphoblastic leukemia

ObjectiveTo investigate the clinical significance and application value of CD304 expression in B-cell acute lymphoblastic leukemia(B-ALL).MethodsThe expression of CD304 in 32 newly diagnosed B-ALL patients, 5 T-cell acute lymphoblastic leukemia patients and 10 control cases with benign hematologic disease were tested by flow cytometry(FCM); 21 specimens in B-ALL patients with co-positive BCR-ABL and CD304 after treatment were detected for minimal residual disease(MRD) by FCM and PCR, and the coincidence rate was then compared.ResultsThe positive rate of CD304 expression in BCR-ABL1 positive patients was higher than those in E2A-PbX1 and fusion gene-negative patients.CD304 were negative expression in T-ALL(n=5) and control groups(n=10).CD304 was positive in 43.75%(14/32) in newly diagnosed B-ALL cases, including 10 positive in 12 BCR-ABL, 1 positive in TEL-AML1, and 3 positive without fusion gene, while CD304 was negative in 3 E2A-PBX and 2 MLL/AF4 cases.The expression frequency of CD66c in CD304-positive B-ALL was increased(P < 0.05).As for MRD of 21 samples, 11 samples were PCR positive, 10 were FCM positive, 10 were double positive.Compared with PCR, the positive coincidence rare of FCM was 91% and total coincidence rare was 95%.ConclusionsCD304 abnormal expressed in B-ALL has an important clinical value in the diagnosis and monitoring MRD.