Viability and functionality of mesenchymal stromal cells loaded on collagen microspheres and incorporated into plasma clots for orthopaedic application: Effect of storage conditions |
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| Institution: | 1. Harvard Medical School Orthopedic Trauma Initiative, Massachusetts General Hospital, Department of Orthopaedic Surgery, Boston, MA, United States;2. Massachusetts General Hospital, Division of Trauma, Emergency Surgery & Surgical Critical Care, Boston, MA, United States;3. University Medical Center Utrecht, Department of Surgery, Utrecht, The Netherlands;1. Cranfield Forensic Institute, Cranfield University, Defence Academy of the United Kingdom, Shrivenham, SN6 8LA, UK;2. Impact and Armour Group, Centre for Defence Engineering, Cranfield University, Defence Academy of the United Kingdom, Shrivenham, SN6 8LA, UK, now at Defence and Security Accelerator, Porton Down, Salisbury, Wiltshire, SP4 0JQ, UK;3. Institute of Naval Medicine, Alverstoke, Gosport, UK;4. Centre for Simulation and Analytics, Cranfield University, Defence Academy of the United Kingdom, Shrivenham, SN6 8LA, UK;5. Royal Centre for Defence Medicine, Birmingham, UK;1. C.S. of Hand Surgery and Microsurgery, AOU Policlinico of Modena, Modena, Italy;2. I Clinica Ortopedica e Traumatologica, Istituto Ortopedico Rizzoli, Bologna, Italy;3. C.S. of Orthopedic Surgery, Hospital Cardinal Massaia, Asti, Italy;4. University of Modena and Reggio Emilia, Italy;1. Department of Orthopaedics, Qingpu Branch of Zhongshan Hospital, Fudan University, Shanghai 201700, China;2. Department of Orthopaedic Surgery, Shanghai Tenth People’s Hospital Affiliated to Tongji University, Shanghai 200072, China;3. Institute of Bone Tumor Affiliated to Tongji University, School of Medicine, Shanghai 200072, China;1. Department of Orthopaedic Surgery, University of Missouri, Columbia, USA;2. Thompson Laboratory for Regenerative Orthopaedics, University of Missouri, Columbia, USA;3. Division of Laboratory Animal Resources, University of Pittsburgh, Pittsburgh, PA, USA;1. Department of Physical and Rehabilitation Medicine, Stem Cell & Regenerative Medicine Institute, Samsung Medical Center, Republic of Korea;2. Department of Rehabilitation Medicine, Kangbuk Samsung Hospital, Sungkyunkwan University School of Medicine, Republic of Korea;3. Department of Physical Medicine and Rehabilitation, Wooridul Spine Hospital, Republic of Korea |
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| Abstract: | BackgroundThere is evidence showing that human mesenchymal stromal cells (MSC) seeded on collagen microspheres (CM) and incorporated into platelet rich plasma (PRP) clots induce bone formation. For clinical trials it is very important to establish standardization of storage and shipment conditions to ensure the viability and functionality of cellular products. We investigate the effect of storage temperature and time on the viability and functionality of human MSC seeded on CM and included into PRP clots for using in the further clinical application for bone regeneration.MethodsMSC/CM/PRP clots were stored at room temperature (RT), 4 °C and 37 °C for 12 h, 24 h and 48 h. At each period of time, MSC were evaluated for their viability and functionality.ResultsMSC from MSC/CM/PRP clots maintained at RT and 37 °C for 24 h showed a high viability (90%) and maintained their capacity of proliferation, migration and osteogenic differentiation. In contrast, MSC/CM/PRP maintained to 4 °C showed a significant reduction in their viability and migration capacity. MSC from MSC/CM/PRP clots maintained at RT for 24 h induce osteogenesis in the subcutaneous tissues of mice, after four months of transplantation.DiscussionOur results show that MSC incorporated into CM/PRP clots and maintained at RT can be utilized in bone regeneration protocols during the first 24 h after their processing. |
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| Keywords: | Mesenchymal stromal cells Storage Collagen PRP Orthopaedic Bone Regeneration |
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