Consequences of butylated hydroxytoluene in the freezing extender on post-thaw characteristics of stallion spermatozoa in vitro |
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Authors: | Morillo-Rodríguez A Macías-García B Tapia J A Ortega-Ferrusola C Peña F J |
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Affiliation: | Laboratory of Equine Reproduction, Veterinary Teaching Hospital, Cáceres, Spain. |
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Abstract: | Ejaculates from six pure Spanish stallions were split, and one subsample frozen in a commercial extender supplemented with the lipid soluble antioxidant butylated hydroxytoluene (BHT), while the other subsample served as control. After at least 4 weeks of storage, samples were thawed and post-thaw sperm quality analysed: sperm motility and kinematics using a CASA system, membrane and acrosome integrity and mitochondrial membrane potential using flow cytometry. The outcome of cryopreservation varied significantly among stallions. However, the supplementation with 1 mm BHT had no significant effect on any of the sperm parameters evaluated post-thaw. |
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Keywords: | Butylated hydroxytoluene CASA flow cytometry freezing sperm stallion |
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