燧心胶囊对慢性心力衰竭大鼠心功能及脑钠肽的影响

目的以慢性心力衰竭大鼠为研究对象,观察燧心胶囊对大鼠血流动力学的影响,测定大鼠血清中脑钛钠（BNP）含量,以探究燧心胶囊治疗慢性心力衰竭的作用机制。方法将42只大鼠分为假手术组（n=8）、模型对照组（n=7）、地高辛组（n=6）、卡托普利组（n=7）、燧心胶囊低剂量组（n=7）、燧心胶囊高剂量组（n=7）。采用腹主动脉缩窄术建立大鼠慢性心力衰竭模型,连续给药12周后,以Medlab 6系统检测左室收缩压（LVSP）、左室舒张末压（LVEDP）、左室压力最大变化速率（±dp/dtmax）以及心率（HR）;酶联免疫吸附法检测血清BNP浓度。结果与模型对照组比较,燧心胶囊能够有效增加LVSP、±dp/dtmax（P〈0.01）,降低LVEDP（P〈0.01）,同时使血清BNP浓度显著降低（P〈0.01）,以高剂量组更为明显。结论燧心胶囊能够改善慢性心力衰竭大鼠心室收缩和舒张功能,纠正其异常的血流动力学状态,使循环中过度分泌的BNP水平明显降低,其疗效有剂量依赖关系。

Effects of Suixin Capsule on heart function and BNP in chronic heart failure rats

Objective To research the mechanism of Suixin Capsule interfering in chronic heart failure(CHF) rats,by determining hemodynamics in vivo and measuring brain natriuretic peptide(BNP) in rats serum.Methods Partially banding abdominal aortic artery to achieve CHF rats model,42 rats were randomly divided into six groups: sham group(n = 8),control group(n = 7),digoxin group(n = 6),captopril group(n = 7),low-dosage group(n = 7),and high-dosage group(n = 7).The left ventricular systolic pressure(LVSP),left ventricular end-diastolic pressure(LVEDP),±dp/dtmax and heart rate(HR) were determined by Medlab 6 system.BNP levels in rats serum were measured by enzyme linked immunosorbent assay(ELISA).Results Compared with control group,LVSP、±dp/dtmax in low-dosage group and high-dosage group significantly increased(P < 0.01),LVEDP in high-dosage group significantly decreased(P < 0.01),while BNP level in low-dosage group and high-dosage group decreased(P < 0.01).Conclusion Suixin Capsule can strengthen ventricular contractile and diastolic function of CHF rats,normalize abnormal hemodynamics in the heart failure state,and decrease BNP level which overproduced in CHF rats serum with a dose-dependent manner.