趋化生长因子受体4和基质金属蛋白酶-13在喉鳞状细胞癌中的表达及临床意义的研究

目的探讨趋化生长因子受体4（CXCR4）及基质金属蛋白酶-13（MMP-13）在喉鳞状细胞癌中的表达,并研究其临床意义。方法采用免疫组织化学方法和RT-PCR方法检测喉鳞状细胞癌组织及远离肿瘤组织的正常喉黏膜组织中CXCR4和MMP-13的蛋白及mRNA表达。结果 CXCR4和MMP-13在喉癌组织中呈过度表达,而自身正常喉黏膜弱表达或不表达;在肿瘤组织中,CXCR4的表达与患者的年龄、性别无关（P〉0.05）,而与临床分期、肿瘤分化程度、淋巴结转移相关（P〈0.05）;MMP-13的过度表达与肿瘤分化程度、淋巴结转移相关（P〈0.05）。蛋白表达水平情况与基因表达水平相一致。CXCR4和MMP-13在喉鳞状细胞癌的蛋白及mRNA表达水平呈显著正相关。结论 CXCR4和MMP-13可能参与了喉鳞状细胞癌的发生,在喉鳞癌的侵袭和转移中发挥着重要作用,联合检测CX-CR4和MMP-13有望成为喉鳞状细胞癌早起诊断和预后判断的分子指标之一。

Study on the expression and clinical significance of CXCR4 and MMP-13 in laryngeal squamous cell carcinoma

Objective To investigate the expression and clinical significance of CXCR4 and MMP-13 in the laryngeal squamous carcinoma.Methods The expression of CXCR4 and MMP-13 in fresh laryngeal squamous carcinoma tissues and adjacent normal tissues were examined by immunohistochemical staining and RT-PCR.Results The positive expression rate of CXCR4 and MMP-13 in tumor samples was significantly higher than that in normal ones;In tumor samples,the expression of CXCR4 were not associated with age and sex(P > 0.05),while it were higher in clinical stage,depth of invasion and lymph node metastasis(P < 0.05).Over-expression of MMP-13 was significantly correlated with depth of invasion and lymph node metastasis(P < 0.05).The expression of protein and mRNA were at the same level.The expression level of CXCR4 and MMP-13 in the laryngeal squamous carcinoma tissue positively correlated.Conclusion CXCR4 and MMP-13 may be involved in the laryngeal squamous carcinoma,and may play an important role in the invasion and metastasis of laryngeal squamous carcinoma.CXCR4 and MMP-13 play a cooperative role in the process of laryngeal squamous carcinoma.