新乌头碱、乌头碱与次乌头碱在附子不同炮制法中含量变化的实验研究

目的探讨不同炮制方法的炮制品中新乌头碱、乌头碱与次乌头碱含量变化。方法分别采用江西建昌帮法、樟树帮法与《中国药典》2005年版法制备附子炮制品,通过HPLC色谱法测定其中新乌头碱、乌头碱与次乌头碱含量。结果 3种炮制方法的炮制品中新乌头碱、乌头碱与次乌头碱的平均质量分数分别为：建昌帮法：（2.13±0.26）,（6.78±0.18）,（11.26±0.97）μg/g;樟树帮法：（2.35±0.39）,（3.23±0.88）,（8.09±0.92）μg/g;中国药典法：（1.43±0.18）,0,（1.68±0.23）μg/g。结论建昌帮煨制附子中新乌头碱、乌头碱与次乌头碱总含量比另外两种方法的总含量要高,这为建昌帮炮制技术的研究提供了科学根据。

Experimental study of the content of new aconitine,aconitine and hypoaconitine in different processing method of aconite

Objective To explore the content of new aconitine,aconitine and hypoaconitine in products processed by different approaches.Methods Aconite processed products were prepared with Jiangxi Jianchangbang method,Zhangshubang method and method provided in Chinese Pharmacopeia(2005 edition).And the content of new aconitine,aconitine and hypoaconitine in the products were determined with chromatography(HPLC) method.Results Average quality scores of new aconitine,aconitine and hypoaconitine processed with the three methods were as follows: With Jianchangbang method,the contents were(2.13±0.26),(6.78±0.18),(11.26±0.97) μg/g,respectively;with Zhangshubang method,the contents were(2.35±0.39),(3.23±0.88),(8.09±0.92) μg/g,respectively;with method provided in Chinese Pharmacopeia,the contents were(1.43±0.18),0,(1.68±0.23) μg/g,respectively.Conclusion Contents of new aconitine,aconitine and hypaconitine are higher when processed with Jianchang method than processed with the other two methods,which provides scientific evidence for further investigation of Jianchangbang method.