| 大孔树脂纯化野葛总黄酮的工艺研究 |
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| 引用本文： | 陈雅维,王丹丹.大孔树脂纯化野葛总黄酮的工艺研究[J].中国医药导报,2012,9(9):106-108. |
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| 作者姓名： | 陈雅维 王丹丹 |
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| 作者单位： | 河南科技大学化工与制药学院,河南洛阳,471003 |
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| 摘 要： | 目的对野葛提取液中总黄酮的纯化分离进行了研究,旨在促进野葛药用价值的进一步发展。方法通过对5种不同型号的大孔树脂：HPD-750、HPD-600、AB-8、NKA-Ⅱ、ADS-17进行静态吸附解吸试验,筛选出AB-8型树脂进行动态试验,确定最佳纯化工艺条件。结果所得最佳条件为：上样液浓度9.82 mg/mL,上样液体积2BV,洗脱剂乙醇体积分数70%,洗脱pH 5～6,在此条件下吸附率可达91.21%,解吸率可达93.61%。结论 AB-8型树脂有较好的综合性能,可用于野葛提取液总黄酮的纯化。
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| 关 键 词： | 野葛 总黄酮 大孔树脂 纯化 |
Technology research on macroporous resin in separation of total flavonoids from Radix Puerariae |
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| CHEN Yawei
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WANG Dandan.Technology research on macroporous resin in separation of total flavonoids from Radix Puerariae[J].China Medical Herald,2012,9(9):106-108. |
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| Authors: | CHEN Yawei WANG Dandan |
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| Institution: | College of Chemical Engineering and Pharmaceutical Engineering,He′nan University of Science and Technology,He′nan Province,Luoyang 471003,China |
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| Abstract: | Objective To develop further application of Radix Puerariae in medicinal purposes,by studying purification technology of total flavonoids.Methods Static adsorption characteristics of five widely used macroporous resins(HPD-750,HPD-600,AB-8,NKA-Ⅱ,ADS-17) for the separation had been critically evaluated.Dynamic adsorption and desorption experiments were next carried out on AB-8 resin packed column to obtain optimal parameters for separating total flavonoids.Results The optimal conditions were that: sample concentration was 9.82 mg/mL,sample total volume was 2BV,desorption solvent was 70% ethanol,desorption pH was 5-6.The rate of adsorption and desorption could reach to 91.21% and 93.61% separately under these conditions.Conclusion AB-8 type of resin has excellent integrative performances which benefits for purification of total flavonoids from Radix Puerariae. |
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| Keywords: | Radix Puerariae Total flavonoids Macroporous resin Purification |
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