肿瘤转移相关基因1与抑制基因KISS-1在胰腺癌中的表达及意义

目的研究肿瘤转移相关基因1（metastasis associated 1,MTA1）与抑制基因KISS-1在胰腺癌中的表达,探讨其与病理指标的关系。方法应用免疫组化Envision二步法在36例胰腺癌组织、10例癌旁胰腺组织中检测MTA1与KISS-1蛋白的表达情况。结果 MTA1在胰腺癌组织中的阳性表达率为77.8%（28/36）,显著高于癌旁胰腺组织中的表达[20.0%（2/10）]（P〈0.01）;KISS-1在胰腺癌组织中的阳性表达率为38.9%（14/36）,明显低于癌旁胰腺组织的90.0%（9/10）（P〈0.01）。二者的表达均与患者年龄、性别及肿瘤部位无关（P〉0.05）,而与癌组织的分化程度、淋巴结转移及临床分期有关（P〈0.05）。胰腺癌组织中MTA1与KISS-1的表达呈负相关（r=-0.533,P〈0.01）。结论MTA1和KISS-1与胰腺癌的发生、侵袭以及转移有关,其有望成为胰腺癌诊治的重要生物学指标。

Expression and significance of tumor metastasis associated 1 and suppressor gene KISS-1 in pancreatic cancer

Objective To study the expression of tumor metastasis associated 1(MTA1) and suppressor gene KISS-1 in the pancreatic cancer and explores its relationship with the pathological indicators.Methods The immunohistochemical Envision two-step method was used to detect the MTA1 and KISS-1 protein expression in 36 cases of pancreatic cancer tissue and 10 cases of normal paracancer tissue.Results The positive expression rate of MTA1 in pancreatic cancer was 77.8%(28/36),which was significantly higher than the 20.0%(2/10) in the normal paracancer tissue(P < 0.01);The positive expression rate of KISS-1 in pancreatic cancer was 90.0%(9/10),which was significantly lower than the 90.0%(9/10) in the normal paracancer tissue(P < 0.01).The expression of both genes were unrelated to the patient age,gender and tumor location(P > 0.05),but related to the differentiation degree of cancer tissue,lymph node metastasis and clinical stage(P < 0.05).In the pancreatic cancer tissue,the expression of MTA1 and KISS-1 were negatively correlated with each other(r =-0.533,P < 0.01).Conclusion MTA1 and KISS-1 are related to the occurrence,invasion and metastasis of pancreatic cancer and therefore are expected to become important biological indicators in the diagnosis and treatment of pancreatic cancer.