金银花中绿原酸和木犀草苷的薄层色谱鉴别方法研究

目的 建立金银花中绿原酸和木犀草苷的薄层色谱鉴别方法.方法 参照〈中国药典〉2010年版一部附录Ⅵ B试验TLC法,分别考察供试品样品的制备,展开剂的选择,以及显色方法.结果 以水为溶剂热回流提取制备试验样品,以乙酸乙酯-丙酮-甲酸-水（7∶3∶1∶1.2）为展开剂,分别喷以5%的三氯化铝乙醇溶液,至紫外光灯（365 nm）下观察鉴定木犀草苷.喷以1%三氯化铁试液与1%铁氰化钾试液（1∶1）混合溶液的上清液,显色鉴定绿原酸.结论 所建立的方法简便可行,重现性好,可用于金银花中绿原酸和木犀草苷的鉴定.

The study of the TLC identification method of Luteoloside and Chlorogenic Acid in Lonicera japonica Thunb.

Objective To establish the TLC identification method of Luteoloside and Chlorogenic acid in Lonicera japonica Thunb..Methods According to TLC testing in Appendix Ⅵ B of Chinese Pharmacopeia(2010 edition,1 volume),the test sample preparation,the agent of choice and the chromogenic method were researched.Results The TLC conditions were as follows: the developing solvent was mixtured of ethyl acetate-acetone-formic acid-water(7∶3∶1∶1.2) and 5% ethanol solution of aluminum chloride to UV light(365 nm) identification of Luteoloside,1% ferric chloride solution and potassium ferricyanide solution(1∶1) mixture of the supernatant used as color-developing agent identification of chlorogenic acid.Conclusion The method is simple and feasible with good reproducibility and can be used to identification of Luteoloside and Chlorogenic acid in Lonicera japonica Thunb..