家兔喉返神经运动神经元的位置及其性质

本文用辣根过氧化物酶(HRP)、胆碱脂酶(AchE)和脑啡呔(ENK)免疫组化法研究家免喉返神经运动神经元在中枢的位置及其性质。发现:HRP标记细胞主要分布在注射侧的疑核、迷走神经背核。在C_(1,2)节段的背内侧核、面后核以及C_1节段的副神经脊髓核内有少量标记细胞。疑核、迷走神经背核和背内核中有少量AchE—HRP双标记神经元,疑核和背内侧核内可见到ENK—HRP双阳性神经元。这表明家兔喉返神经运动神经元有些是胆碱能神经元和ENK样免疫组化反应阳性神经元。本文对喉返神经运动纤维的周围分布及机能意义进行了讨论。

ON THE LOCATION PROPERTY OF RECURRENT LARYNGEAL MOTONEURONS IN THE RABBIT

The location of the recurrent laryngeal motoneurons was traced with CT-HRP and its property was observed by usimg AchE histochemical and ENK immunohistochemical technique. The present study shows that the recurrent laryngeal motoneurons in the rabbit are situated primarily in the ipsilateral ambiguous nucleus, the dorsal motor nucleus of the vagus, the retrofacial nucleus, the dorsomedial nucleus of C_(1,2) and spinal nucleus of the accessory nerve of C_1. In the ambiguous nucleus, dorsal motor nucleus of the vagus and the dorsomedial nucleus the HRP-AchE double labeled positive neurons, and in the ambiguous nucleus and dorsomedial nucleus the HRP-ENK double labeled positive neurons are found. It suggests that some of the recurrent laryngeal motoneurons in the rabbit should be cholinergic and ENK-like immunoreactive positive neurons. Distribution of the recurrent laryngeal motor fibers and its functional significance is discussed.