(+),(-)和(±)棉酚在雌大鼠抗早孕作用的研究

妊娠第6～9天大鼠,分别ig(±)和(-)棉酚80mg·kg^-1·d^-1和40mg·kg^-1·d^-1,结果有明显的抗早孕作用。然而(+)棉酚40mg·kg^-1·d^-1对大鼠生育无明显影响。(-)棉酚30μg·ml^-1能抑制体外培养黄体细胞孕酮的分泌。(+)棉酚10μg·ml^-1能促进黄体细胞分泌孕酮。hCG1IU·ml^-1能明显刺激体外培养颗粒细胞孕酮的分泌。(±)棉酚10和30μg·ml^-1皆能明显降低颗粒细胞对hCG的反应性。

EFFECT OF(+),(-)AND(±)GOSSYPOL ON EARLY PREGNANCY IN RATS

Treatment of rats with(±)gossypol 80 mg·kg^-1 or(-)gossypol 40mg·kg^-1 on day6～9 of gestation terminated early pregnancy.However,(+)gossypol at thedosage of 40 mg·kg^-1 was found to have no effect on gestation,(-)Gossypol at a concentration of30μg·ml^-1 showed an inhibitory effect on progesterone preduction and(+)gossypol at 10μg· ml^-1 caused an increase of progesterone secretion by luteal cells in vit To,A marked increase ofprogesterone level in granulosa cells was elicited by hCG,The steroidogenic response of granulosa cellsto hCG was inhibited by(±)gossypol at 10 and 30μg·ml^-1。