EFFECTS OF TGF-B1 ON THE EXPRESSION OF PLASMINOGENACTIVATOR INHIBITOR TYPE 1 IN CULTURED HUMANRENAL INTERSTITIAL FIBROBLASTS

Objective To investigate the effects of transforming growth factor-β1(TGF-β1) on the expression of plas minogen activator inhibitor type 1 (PAI-1) mRNA in renal interstitial fibrosis in vitro. Methods Human renal interstitial fibroblasts were isolated and cultured in vitro. The cells were stimulated by TGF-β1 with different concentration (0 to 10ng/ml) at different time (0 to 48h). The expression of PAI-1 mRNA was assayed by RT-PCR. Results TGF-β1 had dose-dependent and time-dependent effects on the expression of PAI-1 mRNA in renal interstitial fibroblasts. Conclusion TGF-β1 may partic ipate in renal fibrosis with inducing the expression of PAI-1 mRNA in renal fibroblasts and affecting the synthesis and degradation of extracellular ma trix (ECM) .