血小板生成素注射用于小鼠肿瘤辅助性治疗的初步研究

本文利用ＴＰＯ基因注射治疗接受环磷酰胺化疗后小鼠的血小板及白细胞减少症。将克隆于ｐｃＤＮＡ３的ＴＰＯ质粒按不同剂量注射到试验组２７只小鼠后肢内侧肌肉内，其中３只小鼠注射空质粒。三日后其中接受ＴＰＯ基因注射的１２只小鼠每只腹腔内注射环磷酰胺２ｍｇ（１００ｍｇ／ｋｇ体重），其余１２只不化疗的小鼠作为基因对照组；另设化疗对照组动物１５只，按相同剂量注射环磷酰胺。每周尾静脉采血，检测白细胞和血小板水平。结果基因＋化疗组的两项血液指标在化疗后两周内明显优于单纯化疗组，无毛发脱落；化疗组和注射空质粒组动物绝大多数发生脱毛现象，并有７只动物死亡。两周后进行第二次环磷酰胺攻击，结果化疗组的死亡数达８只，存活动物体况很差，基因＋化疗组动物尚无任何不良表现。此时两组的血液指标已无明显差异，提示在基因注射后ＴＰＯ的有效表达期在２－３周。本研究发现ＴＰＯ基因注射后，不但动物血小板和白细胞水平提高，而且其它化疗后的不良症状也得到改善，提示ＴＰＯ有着更为广泛的作用

Mice Resistant to Cyclophosphomide Toxicity by TPO Gene Injection

We first report that TPO gene injection could prevent mice from leukocytepenia,thrombpenia and alopecia after cyclophosphomide treatment.TPO gene cloned in pcDNA3 was injected 12 mice hind leg muscle,3 mice injected wild pcDNA3 plasmid ,followed with cyclophosphomide treatment(100mg/kg/day,3times) three days later.The number of leukocyte and platelet in gene-injected mice remained higher than controls,and no other symptoms were observed,Meanwhile,most of the controls and these received wild plasmid were hair loss(15/18)and in low spirit ,8 of them died in the second week of cyclophosphomide administer.The hematopoietic activity was independent upon the dose of TPO gene in the range of 20ug to 100ug.In addition ,TPO gene injection seems no harm to mice reproduction.Second cyclophosphomide challenge was made in the 18th day post gene injection,and then no more hemalogical difference was detected between the trials and controls .It suggested that effectiveness fo TPO expression of single injection is about 2to 3 weeks .TPO gene therapy could reduce the toxicity of cyclophosphomide to blood and pevent mice from chemotherapy-induced alopecia.It has great value in cancer accessary treatment.