| 人黑素瘤细胞A375 MIA/CD-RAP基因启动子的克隆及其鉴定 |
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| 引用本文： | 陈思远,黄长征,钱悦,涂亚庭.人黑素瘤细胞A375 MIA/CD-RAP基因启动子的克隆及其鉴定[J].中国麻风皮肤病杂志,2006,22(8):633-635. |
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| 作者姓名： | 陈思远 黄长征 钱悦 涂亚庭 |
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| 作者单位： | 华中科技大学同济医学院附属协和医院皮肤科,武汉,430022 |
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| 摘 要： | 目的:克隆MIA/CD-RAP的启动子序列并对其进行酶切和DNA测序鉴定。方法:从培养的人黑素瘤细胞A375基因组DNA中扩增出MIA/CD-RAP启动子序列,并将其克隆到载体pGL2-Ba-sic上,再经酶切及PCR扩增鉴定和测序。结果:酶切电泳和DNA测序结果表明,已成功克隆了人黑素瘤MIA/CD-RAP启动子序列。结论:人黑素瘤MIA/CD-RAP启动子序列的克隆,可为进一步研究MIA/CD-RAP基因的表达调控奠定基础。
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| 关 键 词： | 黑素瘤抑制蛋白 启动子 克隆 黑素瘤 人 |
| 收稿时间： | 2005-10-21 |
| 修稿时间： | 2005-12-22 |
Cloning of hunan MIA/CD - RAP promoter and its identification |
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| Chen Siyuan, Huang Changzheng, Qian Yue,et al..Cloning of hunan MIA/CD - RAP promoter and its identification[J].China Journal of Leprosy and Skin Diseases,2006,22(8):633-635. |
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| Authors: | Chen Siyuan Huang Changzheng Qian Yue |
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| Institution: | Department of Dermatology, College, Huazhong University of Science and Technology, Wuhan, 430022 |
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| Abstract: | Objective: To clone and identify the MIA/CDRAP promoter sequence in melanoma.Methods: MIA/CD-RAP promoter of human melanoma was amplified by PCR and cloned into vector pGL2-Basic,which was cut out by restriction enzymes,and sequenced.Results: The recombinant plasmid of human MIA/CD-RAP promoter was successfully constructed and identified by PCR and DNA sequencing.(Conclusion): Cloning of human MIA/CD-RAP promoter can form a base for the investigation of expression regulation of MIA/CD-RAP in melanoma in future. |
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| Keywords: | melanoma inhibitory activity promoter clone melanoma human |
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