不同产地甘草有效成分含量分析

目的对不同产地甘草中甘草酸、甘草苷以及甘草总皂苷进行含量测定。方法采用高效液相色谱法测定甘草酸、甘草苷的含量。色谱柱为Thermo sentifi c ODS-2 Hypersil,流动相为乙腈-0.05%磷酸;以甘草酸单铵盐为对照品,香草醛-高氯酸为显色剂,比色法测定甘草总皂苷含量。结果测得不同产地不同种植方式甘草中甘草酸和甘草苷含量符合2010年版药典标准。结论不同产地以及不同种植方式的甘草中,甘草酸、甘草苷及甘草总皂苷的含量差异较大,可为甘草规范化种植提供依据。

Content of active ingredient in Glycyrrhiza uralensis Fisch. from different origins

Objective To determine the content of glycyrrhizic acid, liquiritin and total saponins in Glycyrrhiza uralensis Fisch. from different areas. Methods HPLC method was used to determine the content of liquiritin and glycyrrhizic acid. The column was Thermo sentific ODS-2 Hypersil. The mobile phase consisted of acetonitrile-0.05% phosphoric acid. Mono-ammonium glycyrrhetate was used as the reference, vanillin-perchloric acid was used as the chromogenic agent, and UV was used to analyze the otal saponins. Results The content of liquiritin and glycyrrhizic acid in Glycyrrhiza uralensis Fisch. met with the requirements of 2010 version of pharmacopoeia standards. Conclusion Different origins or planting methods has obvious effect on the conten of liquiritin, glycyrrhizic acid and total saponins in Glycyrrhiza uralensis Fisch., so this experiment may provide a basis for the standard planting of Glycyrrhiza uralensis Fisch..