抗骨桥蛋白抗体对沙鼠肝多房棘球蚴组织周围血管生成的影响

目的观察抗骨桥蛋白（osteopontin,OPN）抗体对长爪沙鼠肝多房棘球蚴（俗称泡球蚴）组织周围血管生成的影响。方法 90只长爪沙鼠随机分为3组,即模型对照组（A组）,兔血清对照组（B组）和抗OPN抗体干预组（C组）,所有沙鼠均采用开腹肝穿刺法接种泡球蚴原头节悬混液0.1ml（约含原头节400个）。B组于接种当天注射兔血清0.15ml/鼠,1次/2d,连续7次,之后改为每周1次,直至处死;C组采用相同的方法注射抗OPN抗体（效价1∶32）;A组不作任何处理。分别与感染后第20、60、100、140、180d每组各处死6只沙鼠,留取肝泡球蚴组织,制作组织切片采用苏木素-伊红（H-E）法和免疫组化Envision法观察各组沙鼠肝泡球蚴组织MVD-CD34的表达情况。结果感染泡球蚴沙鼠肝脏中均可见团块状泡球蚴组织,部分播散至腹腔。感染20d时A、B、C组MVD分别为（9.83±3.87）/HP,（9.67±2.94）/HP和（7.50±1.87）/HP,感染60d时分别为（33.67±3.67）/HP,（32.83±6.11）/HP和（24.33±5.61）/HP,感染100d时分别为（44.67±4.92）/HP,（42.20±6.26）/HP和（28.00±8.76）/HP,感染140d时分别为（34.17±3.19）/HP,（31.67±4.97）/HP和（20.50±4.72）/HP;感染180d时分别为（32.33±7.42）/HP,（29.67±3.88）/HP和（13.50±3.21）/HP。其中感染60d及以后各时间点C组与A组和B组相比,微血管数差异有统计学意义（P〈0.05）。结论抗OPN抗体可抑制沙鼠肝泡球蚴组织周围血管生成。

Effect of anti-osteopontin antibody on angiogenesis of hepatic alveolar hydatid tissue in gerbil

Objective To observe the effect of anti-osteopontin antibody on angiogenesis of hepatic alveolar hydatid tissue in gerbil. Methods Ninety gerbils were infected with echinococcus protoscoleces（approximately 400 for each gerbil）by abdominal opening inoculation in liver.The gerbils were randomly divided into three groups：model group（group A）,rabbit serum injection group（group B）,anti-osteopontin antibody experiment group（group C）.Each gerbil in group C was injected with anti-osteopontin antibody（0.15ml/gerbil,Titer：1∶32）as follows：qod 7times,then,one times each week.Each gerbil in group B was injected with rabbit serum in the same way.Gerbils in group A received no treatment.Six gerbils from each group were sacrificed at 20,60,100,140,and 180 days post-infection,respectively.The liver tissues with hydatid cysts were collected,and the expression of MVD-CD34 was observed by immunohistochemistry staining（Envision method）. Results Infected echinococcus metacestode protoscolexes gerbils liver was observed Hepatic Alveolar Hydatid Tissue,some spread to the abdominal cavity.At 20 days post-infection,the number of microvessels of group A,group B,group C respectively presented as follows：（9.83±3.87）/HP,（9.67±2.94）/HP and（7.50±1.87）/HP.And at each other 40 days timepoints,the number of microvessels of each groups were（33.67±3.67）/HP,（32.83±6.11）/HP and（24.33±5.61）/HP;（44.67±4.92）/HP,（42.20±6.26）/HP and（28.00±8.76）/HP;（34.17±3.19）/HP,（31.67±4.97）/HP and（20.50±4.72）/HP;（32.33±7.42）/HP,（29.67±3.88）/HP and（13.50±3.21）/HP.At60,100,140 and 180days post-infection,the microvesseldensity（MVD）of Hepatic Alveolar Hydatid Tissue of group C was lower than that of group A and group B（P〈0.05）. Conclusion The anti-osteopontin antibody can suppresses the angiogenesis of hepatic Alveolar Hydatid Tissue in Gerbil.