| Inhibition Effect of Cyclosporin A on Human Retinal Pigment Epithelial Cells in Vitro |
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| 作者姓名： | Zhaoyan Wang Yannian Hui |
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| 作者单位： | Zhaoyan Wang，Yannian Hui，Ming Hu
Eye Center，Xijing Hospital，4th Military Medical University，Xian 710032，China |
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| 基金项目： | The project was supported by the national natural science foundation of China (No.39470742) |
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| 摘 要： | Purpose: To select effective drugs against cellular proliferation in the vitreous. Methods: Cyclosporin A (0. 125mg/l - 4.0mg/l) was added to cultures of human retinal pigment epithelial (RPE) cells with or without macrophage - conditioned medium (MCM) . Proliferation rate of the cells was measured with (3H) - thymidine incorporation and liquid scintillation techniques on days 3 and 5.Results: Cyclosporin A at a dosages of 0. 125mg/l had a slight inhibition on human RPE cells proliferation (-3.8%-4.1%, P>0.05).Cyclosporin A at a dose ranging from 0.25mg/l to 4.0mg/l inhibited cellular proliferation effectively and in a dose - dependent manner (8.7% -95.1%, P<0.05 r=0.94, P<0.001) with its ID50 of 1.49mg/l. In the culture of RPE with MCM, the inhibition on day 5 was more effective than that on day 3. Conclusion : Cyclosporin A had an effective inhibition on human RPE cell proliferation and it may be of potential use clinically. Eye science 1998; 14 : 41 - 44.
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| 关 键 词： | CSA 视网膜 色素上皮细胞 抑制作用 |
Inhibition Effect of Cyclosporin A on Human Retinal Pigment Epithelial Cells in Vitro |
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| Zhaoyan Wang,Yannian Hui,Ming Hu
Eye Center,Xijing Hospital,th Military Medical University,Xian ,China.Inhibition Effect of Cyclosporin A on Human Retinal Pigment Epithelial Cells in Vitro[J].Eye Science,1998,14(1):41-44. |
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| Authors: | Zhaoyan Wang Yannian Hui Ming Hu
Eye Center Xijing Hospital th Military Medical University Xian China |
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| Institution: | Eye Center, Xijing Hospital, 4th Military Medical University, Xian 710032, China. |
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| Abstract: | Purpose: To select effective drugs against cellular proliferation in the vitreous. Methods: Cyclosporin A (0. 125mg/l - 4.0mg/l) was added to cultures of human retinal pigment epithelial (RPE) cells with or without macrophage - conditioned medium (MCM) . Proliferation rate of the cells was measured with (3H) - thymidine incorporation and liquid scintillation techniques on days 3 and 5.
Results: Cyclosporin A at a dosages of 0. 125mg/l had a slight inhibition on human RPE cells proliferation (-3.8%-4.1%, P>0.05).Cyclosporin A at a dose ranging from 0.25mg/l to 4.0mg/l inhibited cellular proliferation effectively and in a dose - dependent manner (8.7% -95.1%, P<0.05 r=0.94, P<0.001) with its ID50 of 1.49mg/l. In the culture of RPE with MCM, the inhibition on day 5 was more effective than that on day 3. Conclusion : Cyclosporin A had an effective inhibition on human RPE cell proliferation and it may be of potential use clinically. Eye science 1998; 14 : 41 - 44. |
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| Keywords: | cyclosporin A retinal pigment epithelium ~3H - TdR |
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