葛根总黄酮诱导NB4细胞凋亡的机制探讨

目的 探讨葛根总黄酮(PR)对人早幼粒细胞白血病(APL)NB4细胞株的影响.方法 MTT法检测NB4细胞增殖抑制率;FITC-Annexin V/PI 双染法检测细胞凋亡率;Western blot检测c-Jun氨基端激酶(JNK)、Bcl-2、PARP、半胱天冬酶(Caspase)3蛋白表达的变化.结果 PR呈时间-剂量依赖性抑制NB4细胞增殖,诱导细胞凋亡;JNK、PARP及Caspase 3的表达与PR浓度呈正相关,而与凋亡抑制蛋白Bcl-2呈负相关.结论 PR可有效诱导NB4细胞凋亡,其作用与JNK信号途径的活化有关.

A study on the mechanism of apoptosis of NB4 cells induced by flavonoids of puerarin

Objective To explore the effects and the possible molecular mechanism of flavonoids of puerarin (PR) on acute promyelocytic leukemia (APL) cell line NB4 cells in vitro.Methods MTT assay was used to detect the inhibitory effects of cell proliferation.The apoptosis of NB4 cells were detected by flow cytometry marked with Annexin V/PI.The expressions of JNK,PARP,Bcl-2 and Caspase 3 were detected by Western blot.Results PR could inhibit the proliferation of NB4 cells in time-and dose-dependent manner.The protein expressions of JNK,PARP and Caspase 3 were possitively correlated to the concentration of PR,but negetively correlated to Bcl-2.Conclusion It is suggested that PR may be a potential anti-leukemia agent that could induce NB4 cells apoptosis and activate JNK signaling pathway.