| 葛根素对巨噬细胞基质金属蛋白酶9及其组织抑制物1表达的影响 |
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| 引用本文： | 曹喆,胡文志,孙烈,杨季明,张博晴,周海波.葛根素对巨噬细胞基质金属蛋白酶9及其组织抑制物1表达的影响[J].江苏医药,2010,36(3). |
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| 作者姓名： | 曹喆 胡文志 孙烈 杨季明 张博晴 周海波 |
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| 作者单位： | 南京医科大学第二附属医院心血管病科,210011 |
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| 摘 要： | 目的 观察葛根素对巨噬细胞分泌和表达基质金属蛋白酶9(MMP-9)及其组织抑制因子1(TIMP-1)的影响.方法 将人单核细胞系THP-1来源的巨噬细胞与不同浓度的葛根素进行培养,采用RT-PCR和ELISA方法 分别检测巨噬细胞的MMP-9、TIMP-1的基凶和蛋白质表达.结果 葛根素对氧化低密度脂蛋白(ox-LDL)诱导THP-1细胞MMP-9 mRNA及其蛋白质表达作用呈浓度依赖性地减少MMP-9 mRNA及其蛋白的表达,但对TIMP-1的表达无影响.结论 葛根素可以通过调节巨噬细胞分泌MMP-9途径发挥稳定动脉粥样硬化斑块的作用.
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| 关 键 词： | 葛根素 巨噬细胞 基质金属蚩白酶9 金属蛋白酶组织抑制物1 |
Effect of puerarin on expression of matrix metalloproteinase-9 and matrix metalloproteinase tissue inhibitor-1 in THP-1 monocyte-derived macrophages Induced by oxidized low density lipoprotein |
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| Abstract: | Objective To investigate the effect of puerarin on expression of matrix metalloproteinase-9(MMP-9) and matrix metalloproteinase tissue inhibitor-1 (TIMP-1) in THP-1 monocyte-derived macrophages induced by oxidized low density lipoprotein(oxLDL). Methods THP-1 monocyte-derived macrophages induced by ox-LDL were cultured with different concentrations of puerarin and the expressions of MMP-9 arid TIMP-1 were analysed by RT-PCR and ELISA. Results Puerarin inhibited the expression of MMP-9 concentration-dependently, but had no effect on the expression of TIMP-1. Conclusion Puerarin can make atheroseclerotic plaque stable by regulating the expression of MMP-9 in the macrophages. |
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| Keywords: | Puerarin Maerophages Matrix metalloproteinase-9 Matrix metalloproteinase tissue inhibitor-1 |
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