| 前列腺癌中基质金属蛋白酶7和9的表达 |
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| 引用本文： | 张莉. 前列腺癌中基质金属蛋白酶7和9的表达[J]. 实用医技杂志, 2011, 18(3): 236-238 |
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| 作者姓名： | 张莉 |
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| 作者单位： | 山西医科大学,030001 |
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| 摘 要： | 目的探讨基质金属蛋白酶（MMP）-7,-9在前列腺癌转移侵袭中的作用,以及血管内皮生长因子（VEGF）与MMPs的关系。方法采用免疫组织化学和形态定量分析方法分析MMP-7及MMP-9表达情况,同时培养PC3M细胞并采用Boyden小室侵袭试验及酶谱分析观察MMP-9及其与VEGF在前列腺癌中的作用。结果前列腺癌各分级中MMP-7和MMP-9的表达差异均有统计学意义（P〈0.01）。Boyden小室侵袭实验显示,不同试验组穿透Matrigel的细胞数依次递减,即PC3M〉PC3M＋MMP-9Ab（1∶100）〉PC3M＋MMP-9Ab（1∶50）,差异均有统计学意义（P〈001）。酶谱分析观察发现,直接培养于培养皿表面的PC3M细胞仅有稳定的MMP-9和MMP-2酶原表达,但PC3M细胞与VEGF共孵育组条件培养基中MMP9酶原含量以VEGF剂量依赖的方式增加。结论 MMP-7和MMP-9可作为肿瘤侵袭和转移的判定指标。VEGF与MMPs的分泌有关,其机制有待于进一步探讨。
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| 关 键 词： | 前列腺肿瘤 基质金属蛋白酶 血管内皮细胞生长因子 |
Expression of the matrix metalloproteinases 7 and 9 in the prostate cancer |
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| ZHANG Li. Expression of the matrix metalloproteinases 7 and 9 in the prostate cancer[J]. Journal of Practical Medical Techniques, 2011, 18(3): 236-238 |
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| Authors: | ZHANG Li |
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| Affiliation: | ZHANG Li.Shanxi Medical Univer-sity,Taiyuan 030001,China |
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| Abstract: | Objective To investigate the expression of the matrix metalloproteinases(MMP)-7 and-9 in the pro-state cancer,and the relationship of between the MMP and vascular endothelial growth factor(VEGF).Methods The expression of the MMP 7 and 9 were detected by immunohistochemistry assay and morphology quantitative analysis technique.At the same time,the PC3 M cells were cultured,the expression of MMP-9 and the relationship between MMP-9 and VEGF were detected with Boyden chamber and zymography assay.Results The ex... |
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| Keywords: | Pprostate gland cancer Matrix metalloproteinase Vascular endothelial growth factor |
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