| 腺相关病毒介导绿色荧光蛋白基因体外转染人羊膜上皮细胞 |
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| 引用本文： | 罗宏武,黄湘俊,刘浔阳,黄飞舟.腺相关病毒介导绿色荧光蛋白基因体外转染人羊膜上皮细胞[J].中国组织工程研究与临床康复,2010,14(10). |
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| 作者姓名： | 罗宏武 黄湘俊 刘浔阳 黄飞舟 |
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| 作者单位： | 中南大学湘雅三医院肝胆外科,湖南省长沙市,413000 |
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| 摘 要： | 背景:人羊膜上皮细胞易于获得,采集简单,是细胞移植和组织修复的理想种子细胞,目前国内外尚无关于标记、示踪体外培养的人羊膜上皮细胞的报道.目的:探讨腺相关病毒载体介导绿色荧光蛋白摹因对体外培养的人羊膜上皮细胞的转染效果.方法:取人羊膜标本,以胰蛋白酶消化法分离培养人羊膜上皮细胞,采用含绿色荧光蛋白的腺相关病毒进行转染,检测其转染效率.结果与结论:人羊膜上皮细胞可成功地在体外进行原代和传代培养,经含绿色荧光蛋白的腺相关病毒颗粒转染后,可稳定高效表达绿色荧光蛋白,转染效率达58%.
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| 关 键 词： | 腺相关病毒 绿色荧光蛋白 基因 转染 人羊膜上皮细胞 |
Adeno-associated virus vector-medicated green fluorescent protein transfected human amniotic epithelial cells in vitro |
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| Luo Hong-wu,Huang Xiang-jun,Liu Xun-yang,Huang Fei-zhou.Adeno-associated virus vector-medicated green fluorescent protein transfected human amniotic epithelial cells in vitro[J].Journal of Clinical Rehabilitative Tissue Engineering Research,2010,14(10). |
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| Authors: | Luo Hong-wu Huang Xiang-jun Liu Xun-yang Huang Fei-zhou |
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| Institution: | Luo Hong-wu,Huang Xiang-jun,Liu Xun-yang,Huang Fei-zhou Department of Hepatobiliary Surgery,Third Xiangya Hospital,Central South University,Changsha 413000,Hunan Province,China |
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| Abstract: | BACKGROUND: Human amniotic epithelial cells (AECs) are easy to obtain and can function as ideal seed cells for cell transplantation and tissue repair. Currently, marking and tracing of human AECs remains rarely reported. OBJECTIVE: To explore the efficiency of adeno-associated virus (AAV) vector-medicated green fluorescent protein (GFP) on in vitro cultured human AECs transfection. METHODS: Human amnion samples were harvested and trypsinized to isolate human AECs. The AECs were transfected with AAV-GFP, and... |
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