黄芩苷对海人酸致痫小鼠海马x染色体连锁凋亡抑制蛋白表达的影响

目的探讨黄芩苷对海人酸诱导的小鼠癫痫持续状态后海马组织x染色体连锁凋亡抑制蛋白（XIAP）表达的影响。方法将90只ICR雄性小鼠随机分为对照组、癫痫持续状态（sE）组、黄芩苷治疗组，每组30只；采用侧脑室注入海人酸建立小鼠癫痫持续状态模型。通过苏木素一伊红（HE）染色观察黄芩苷对小鼠癫痫持续状态后海马神经细胞的形态学改变；应用免疫组化和Westernblot方法检测小鼠海马组织中XIAP的表达量。结果黄芩苷明显改善sE后小鼠海马组织的病理形态学，海马CA3区XIAP在sE后6h起逐渐增加，12h达高峰，24h有所下降；与对照组比较，差异有统计学意义（P〈0．01）。黄芩苷治疗组海马XIAP蛋白表达在6h、12h和24h均高于sE组（P〈0．05）。结论黄芩苷对小鼠癫痫持续状态后海马神经细胞的保护作用，可能与其促进XIAP的表达有关。

Effect of baicalin on expression of X-linked inhibitor of apoptosis protein in hippocampus of mice after kainic acid-induced status epilepticus

Objective To investigate the effect of baicalin on the expression of X-linked inhibitor of apoptosis protein （XIAP） in the hippocampus of mice after kainic acid-induced status epilepticus （SE）. Methods Ninety male ICR mice were randomly and equally divided into three groups ： control, SE, and baicalin treatment groups. A mouse model of SE was established by injection of 0.1 p~g/ 5 txl kainic acid into the lateral ventricle. HE staining was used to observe the pathological changes in hippocampal CA1 and CA3 re- gions after SE; the expression of XIAP was measured by immunohistochemistry and Western blot. Results Baicalin significantly re- duced the pathological changes in the hippocampus of mice after SE. In the SE group, the expression of XIAP in hippocampal CA1 and CA3 regions increased gradually since 6 hrs after SE, reached the peak level at 12 hrs, and decreased at 24 hrs; the expression of XI- AP was significantly higher in the SE group than in the control group at the three time points （P 〈 0.01 ） ; compared with the SE group, the baicalin treatment group had significantly higher expression of XIAP in hippocampal CA1 and CA3 regions at 6, 12, and 24 hrs （P 〈 0.05）. Conclusions Baicalin has a protective effect on the hippocampal neurons in mice after SE, which might be associated with the up-regulated expression of XIAP.