五味子不同炮制品对小鼠脾淋巴细胞增殖的影响

目的:比较五味子不同炮制品对小鼠脾淋巴细胞增殖作用的影响,为五味子临床合理用药提供实验依据。方法:将五味子不同质量浓度(400,200,100,50 mg·L-1)提取液与细胞密度为4×106个/m L的脾淋巴细胞于96孔板中共同培养48 h,每组6个复孔,以增殖率为指标,采用CCK-8比较生五味子、酒五味子、醋五味子对小鼠脾淋巴细胞、刀豆蛋白A(Con A)和脂多糖(LPS)诱导的T,B淋巴细胞增殖的影响。结果:与空白组比较,Con A,LPS可诱导T,B淋巴细胞增殖;与不同指标相应空白组比较,五味子给药组均能不同程度促进未活化脾淋巴细胞及Con A和LPS诱导的T,B淋巴细胞的增殖,同等剂量下,酒五味子增殖效果最佳,优于生五味子、醋五味子(P0.05,P0.01)。结论:五味子酒制后对小鼠淋巴细胞增殖作用显著增强,符合"入补药熟用"传统理论。

Effect of Schisandrae Chinensis Fructus Prepared with Different Processing Methods on Splenic Lymphocyte Proliferation in Mice

Objective: To compare Schisandrae Chinensis Fructus prepared with different processing methods on splenic lymphocyte proliferation in mice of. Method: The splenic lymphocyte cells were cultured with different concentrations(400,200,100,50 mg · L^-1) of Schisandrae Chinensis Fructu extraction in 96-well plate, each concentration was repeated for six times. After 48 hours, the cell proliferation rate of spleen cells and T/B lymphocytes were measured by the CCK-8 colorimetric method.The T lymphocyte was induced by concanavalin A (Con A)and B lymphocyte was induced by lipopolysaccharide (LPS). Result: Compared with the blank group,Con A, LPS could induce T lymphocyte and B lymphocyte. Compared with blank group in terms of other corresponding indicators, different concentrations of Schisandrae Chinensis Fructus extraction could increase proliferation rate, especially in wine steaming Schisandrae Chinensis Fructus group with the same dosage, wine steaming Schisandrae Chinensis Fructus was better than crude Schisandrae Chinensis Fructus and vinegar steaming Schisandrae Chinensis Fructs. Conclusion: Wine steaming Schisandrae Chinensis Fructus could obviously increase the cell proliferation rate, the result is accordance with TCM theory of processed Schisandra Chinensis Fruits as Tonic.