庆大霉素和卡那霉素耳中毒后雏鸡耳蜗毛细胞的再生

给２大龄纯种罗丝鸡分别肌注庆大霉素（ＧＭ）和卡那霉素（ＫＭ）１０天，于停药当天、第１、２、３和４周处死小鸡，取出内耳基底乳头（ＢＰ）用扫描电镜和光镜观察，发现从ＢＰ的基底到中部有广泛性损害，毛细胞损失率约占４０％，在１周后受损部位表皮板上出现丛生的有微绒毛的再生毛细胞，随恢复期延长，再生毛细胞表面积增大，动纤毛和静纤毛来长出，３至４周受损部位已完全被发育成熟的再生毛细胞填补，ＢＰ形态恢复。

Hair Cell Regeneration and Recovery Following Gentamicin Induced Damage in the Chick Coclea

Two-day old chicks were given gentamicin(GM)and kanamycin(KM)for 10 days and then the cochleas were processed for both scanning electron microscopy and light microscopy on the same day and in 1,2,3,and 4 weeks following the last injection to examine hair cells regeneration and recovery.The result showed that hair cell loss was up to 40% in the base of the basilar papilla following GM and KM administration. One week after injection,however, most of the damaged region was replaced with regenerated hair cells.Three to four weeks later, the regenerated hair cells were developed into normal sensory hair cells. This experiment demonstrated that the hair cells in the chick cochlea were able to re-generate and recover following GM and KM induced damage.