| 不同生长期的叶下珠中槲皮素含量比较 |
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| 引用本文： | 王琳,朱缨. 不同生长期的叶下珠中槲皮素含量比较[J]. 中国药业, 2010, 19(21): 6-7 |
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| 作者姓名： | 王琳 朱缨 |
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| 作者单位： | 苏州卫生职业技术学院,江苏,苏州,215009 |
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| 基金项目： | 苏州卫生职业技术学院院级课题;项目 |
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| 摘 要： | 目的采用高效液相色谱法测定不同生长期的叶下珠中槲皮素的含量。方法色谱柱为Zorbax SB-C18柱（250mm×4.6mm,5.0μm）,流动相为乙腈-0.2%磷酸（30：70）,流速为1mL/min,紫外检测波长为370nm。结果槲皮素进样量在0.05～0.40μg范围内与峰面积具有良好的线性关系,r=0.9999（n=8）,平均回收率为102.54%,RSD=1.74%（n=6）。结论 9月至10月采收的叶下珠中槲皮素的含量最高。
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| 关 键 词： | 叶下珠 槲皮素 含量 |
Determination of Quercetin in Phyllanthus Urinaria L. from Different Growth Periods |
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| Wang Lin,Zhu Ying. Determination of Quercetin in Phyllanthus Urinaria L. from Different Growth Periods[J]. China Pharmaceuticals, 2010, 19(21): 6-7 |
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| Authors: | Wang Lin Zhu Ying |
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| Affiliation: | (Suzhou Health College,Suzhou,Jiangsu,China 215009) |
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| Abstract: | Objective To develop an HPLC method to determine the contents of quercetin in Phyllanthus urinaria L.from different growth periods.Methods The extracts were seperated on Zorbax SB-C18 column（250 mm × 4.6 mm,5.0 μm） with acetonitrile-0.2% phosphoric acid（30：70） as the mobile phase.The flow rate was 1 mL /min.The detection wavelength was at 370 nm.Results The method was linear within the range of 0.05-0.40 μg（r = 0.999 9）.The average recovery rate was 102.54%,RSD was 1.74%（n = 6）.Conclusion Phyllanthus urinaria L.grown in September and October is found to contain the highest contents of quercetin. |
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| Keywords: | Phyllanthus urinaria L. quercetin content |
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